Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1/phospholipase C-γ1 signaling pathway in piglets

作     者：Guangmang Liu Xiaomei Xu Caimei Wu Gang Jia Hua Zhao Xiaoling Chen Gang Tian Jingyi Cai Jing Wang Guangmang Liu;Xiaomei Xu;Caimei Wu;Gang Jia;Hua Zhao;Xiaoling Chen;Gang Tian;Jingyi Cai;Jing Wang

作者机构：Institute of Animal NutritionSichuan Agricultural UniversityChengdu611130China Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of EducationChengdu611130China Maize Research InstituteSichuan Agricultural UniversityChengdu611130China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第8卷第1期

页      面：135-143页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：This research is supported by the Sichuan Science and Technology Program(No.2020YJ0398) 

主　　题：Spermine Intestinal barrier Rac1/PLC-γ1 signaling pathway Intestinal integrity 

摘      要：Weaning stress can cause tight junctions damage and intestinal permeability enhancement,which leads to intestinal imbalance and growth retardation,thereby causing damage to piglet growth and *** can reduce ***,the mechanism of spermine modulating the intestinal integrity in pigs remains largely *** study aims to examine whether spermine protects the intestinal barrier integrity of piglets through ras-related C3 botulinum toxin substrate 1(Rac1)/phospholipase C-g1(PLC-γ1)signaling *** vivo,80 piglets were categorised into 4 control groups and 4 spermine groups(10 piglets per group).The piglets were fed with normal saline or spermine at 0.4 mmol/kg BW for 7 h and 3,6 and 9 *** vitro,we investigated whether spermine protects the intestinal barrier after a tumor necrosis factor a(TNF-a)challenge through Rac1/PLC-γ1 signaling *** in vivo study found that spermine supplementation increased tight junction protein mRNA levels and Rac1/PLC-γ1 signaling pathway gene expression in the jejunum of *** serum D-lactate content was significantly decreased after spermine supplementation(P0.05).The in vitro study found that 0.1 mmol/L spermine increased the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability(P0.05).Further experiments demonstrated that spermine supplementation enhanced the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability compared with the NSC-23766 and U73122 treatment with spermine after TNF-a challenge(P0.05).Collectively,spermine protects intestinal barrier integrity through Rac1/PLC-γ1 signaling pathway in piglets.