A Bioorthogonal-Activated Fluorescence Turn-On Probe Based on Nitrone-Modified 1,8-Naphthalimide for Live-Cell Imaging

作     者：Yu Teng Hong Yang Xiang Li Yongcheng Wang Dali Yin Yulin Tian Yu Teng;Hong Yang;Xiang Li;Yongcheng Wang;Dali Yin;Yulin Tian

作者机构：State Key Laboratory of Bioactive Substances and Function of Natural MedicineBeijing Key Laboratory of Active Substances Discovery and Drugability EvaluationInstitute of Materia MedicaPeking Union Medical College and Chinese Academy of Medical Sciences1st Xian Nong Tan StreetBeijing 100050China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2022年第40卷第2期

页      面：209-214页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：This work was supported by the Beijing Nova Program(Z201100006820049) the National Natural Science Foundation of China(No.21907109) 

主　　题：Bioorthogonal reaction Fluorescence Naphthalimide Imaging agents Cycloaddition 

摘      要：A nitrone-modified 1,8-naphthalimide was desig ned as a novel bioorthog on alactivated turn-on probe based on strain-promoted alkyne-nitrone cycloadditio n(SPANC).The bioorthog onal cycloadducts were subseque ntly tran sformed into fluoresce nt rearra nge-ment products by photo-accelerati on,which exhibited sign ificant fluoresce nee enhan ceme nt,large stokes shift,and high fluores-cence qua ntum *** calculati ons were performed to elucidate the fluoresce nee OFF-ON mecha *** fluoroge nic strategy was successfully applied to labeling of proteins and visualizing mitochondria in live cells in real time.