Kunming mouse strain is less susceptible to elastase-induced abdominal aortic aneurysms

作     者：Haole Liu Kangli Tian Congcong Xia Panpan Wei Boyu Xu Weilai Fu Yankui Li Yafeng Li Liang Bai Rong Wang Weirong Wang Baohui Xu Enqi Liu Sihai Zhao Haole Liu;Kangli Tian;Congcong Xia;Panpan Wei;Boyu Xu;Weilai Fu;Yankui Li;Yafeng Li;Liang Bai;Rong Wang;Weirong Wang;Baohui Xu;Enqi Liu;Sihai Zhao

作者机构：Institute of Cardiovascular ScienceSchool of Basic Medical SciencesXi'an Jiaotong University Health Science CenterXi'anChina Laboratory Animal CenterXi'an Jiaotong UniversityXi'anChina Department of Vascular SurgeryThe Second Hospital of Tianjin Medical UniversityTianjinChina Pain Rehabilitation Department of TCM Orthopedic CenterXi'an Honghui HospitalXi'anChina Department of Vascular SurgeryStanford University School of MedicineStanfordCaliforniaUSA 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2022年第5卷第1期

页      面：72-80页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：partly supported by grants from the Natural Science Foundation of Shaanxi Province (2020PT-004, 2017BSHQYXMZZ18 and 2021PT-056) the National Natural Science Foundation of China (82070470 and 81370379) 

主　　题：abdominal aortic aneurysms C57BL/6J histology Kunming strain 

摘      要：Background:Porcine pancreatic elastase(PPE)is successfully used to induce abdominal aortic aneurysm(AAA)in ***,differences between mouse strains in susceptibility to PPE induction have been *** mouse is one of the most frequently used strains in China but whether it is suitable for induction of AAA by PPE application remains ***:PPE infusion(1.5 units/ml)in temporary controlled aorta was performed to induce AAAs in both C57BL/6J and Kunming *** saline(PBS)application was used as vehicle *** aorta diameters of all mice were measured at days 0 and 14 after surgery to evaluate the AAA ***:After 14 days of PPE or PBS infusion,all mice were sacrificed and aorta tissues were collected for histological staining *** the 14th day after infusion,PPE successfully induced aortic dilation in Kunming mice and typical AAA in C57BL/6J *** aorta diameter increased by 0.23 mm in Kunming mice after PPE infusion,while it was 0.72 mm in the C57BL/6J *** induced mild elastin degradation,smooth muscle cell(SMC)depletion and mural leucocyte infiltration in Kunming mice,but in PPE-sensitive C57BL/6J mice,it induced total loss of SMCs,elastin disappearance and diffused infiltrated leucocytes in aortic aneurysmal *** effects of PPE in inducing angiogenesis and upregulating matrix metalloproteinase 2 and 9 expression in Kunming mice were also weaker than that in C57BL/6J ***:At the reported dose of PPE,Kunming mouse is not as susceptible to AAA formation as C57BL/6J *** failure of PPE to induce AAA formation in Kunming mice may be associated to its inability to boost a strong inflammatory response.