Key genes and mechanisms underlying natural variation of silique length in oilseed rape(Brassica napus L.)germplasm

作     者：Quaid Hussain Jiepeng Zhan Huabing Liang Xinfa Wang Guihua Liu Jiaqin Shi Hanzhong Wang Quaid Hussain;Jiepeng Zhan;Huabing Liang;Xinfa Wang;Guihua Liu;Jiaqin Shi;Hanzhong Wang

作者机构：Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture and Rural AffairsWuhan 430062HubeiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第3期

页      面：617-626页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：the Technical Innovation Project of Hubei Province(2018ABA087) National Natural Science Foundation of China(31771840) the China Agriculture Research System of MOF and MARA(CARS-13) the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2013-OCRI) Fundamental Research Funds for Central Non-Profit Institute of Crop Sciences CAAS(Y2020YJ09) 

主　　题：Brassica napus Silique length GWAS RNA-seq Phytohormones Cell number Cell size Bna A9.ARF18 Bna A9.CYP78A9 

摘      要：Silique length influences seed yield in oilseed *** shows extensive variation in germplasm resources,and identifying the underlying genes and regulatory mechanisms would advance breeding for the *** the present study,a genome-wide association study(GWAS)using 331 core accessions planted in 10 environments revealed 13 loci associated with silique length on chromosomes A01,A04,A07,A09,and C03,explaining 6.2%–19.2%of phenotypic *** analysis showed that silique length variation was attributable to differences in silique growth rate and/or duration before four weeks after flowering,with levels of endogenous phytohormones(auxin,ethylene,and GA24,GA12,and GA44)playing an important *** analysis showed that silique length variation was due mainly to differences in cell number followed by cell *** analysis of two pools of silique walls with opposite length extremes revealed 3248 differentially expressed genes(DEGs).These DEGs were enriched in several pathways(such as cell wall,cell division,and hormone metabolism)associated with cell proliferation and expansion and silique *** GWAS,RNA-seq,and functional annotation results revealed 15 candidate genes for the major associated locus q *** these,Bna ***18 and Bna ***78 A9 were validated by haplotype analysis followed by candidate gene *** variation in the coding region of Bna ***18 and expression of Bna ***78 A9 in silique walls were strongly associated with silique *** results provide an explanation for the natural variation of silique length in oilseed rape germplasm and offer useful information for its improvement.