Root exudates mediate plant defense against foliar pathogens by recruiting beneficial microbes

作     者：Tao Wen Mengli Zhao Jun Yuan George A.Kowalchuk Qirong Shen Tao Wen;Mengli Zhao;Jun Yuan;George A.Kowalchuk;Qirong Shen

作者机构：Jiangsu Provincial Key Lab for Organic Solid Waste UtilizationNational Engineering Research Center for Organic-based FertilizersJiangsu Collaborative Innovation Center for Solid Organic Waste Resource UtilizationNanjing Agricultural UniversityNanjing 210095China Ecology and Biodiversity GroupDepartment of BiologyInstitute of Environmental BiologyUtrecht UniversityPadualaan 83584 CH UtrechtThe Netherlands 

出 版 物：《Soil Ecology Letters》 (土壤生态学快报（英文）)

年 卷 期：2021年第3卷第1期

页      面：42-51页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0818[工学-地质资源与地质工程] 0903[农学-农业资源与环境] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：the National Natural Science Foundation of China(31902107) Natural Science Foundation of Jiangsu Province(BK20170724) National Postdoctoral Program for Innovative Talents(BX201600075). 

主　　题：Foliar pathogen resistance Pseudomonas Recruitment Root exudates 

摘      要：Plants are capable of releasing specific root exudates to recruit beneficial rhizosphere microbes upon foliar pathogen invasion attack,including long-chain fatty acids,amino acids,short-chain organic acids and sugars.Although long-chain fatty acids and amino acids application have been linked to soil legacy effects that improve future plant performance in the presence of the pathogen,the precise mechanisms involved are to a large extent still unknown.Here,we conditioned soils with long-chain fatty acids and amino acids application(L+A)or short-chain organic acids and sugars(S+S)to examine the direct role of such exudates on soil microbiome structure and function.The L+A treatment recruited higher abundances of Proteobacteria which were further identified as members of the genera Sphingomonas,Pseudomonas,Roseiflexus,and Flavitalea.We then isolated the enriched bacterial strains from these groups,identifying ten Pseudomonas strains that were able to help host plant to resist foliar pathogen infection.Further investigation showed that the L+A treatment resulted in growth promotion of these Pseudomonas strains.Collectively,our data suggest that long-chain fatty acids and amino acids stimulated by foliar pathogen infection can recruit specific Pseudomonas populations that can help protect the host plant or future plant generations.