Combination of oridonin and TRAIL induces apoptosis in uveal melanoma cells by upregulating DR5

作     者：Xin Hua Peng Wu Guo-Sheng Gao Xiao-Lei Ye 

作者机构：Department of Clinical LaboratoryHwa Mei HospitalUniversity of Chinese Academy of SciencesNingbo 315010Zhejiang ProvinceChina Ningbo Institute of Life and Health IndustryUniversity of Chinese Academy of SciencesNingbo 315010Zhejiang ProvinceChina Department of Clinical LaboratoryRenmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina College of Life SciencesChina West Normal UniversityNanchong 637009Sichuan ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2021年第14卷第12期

页      面：1834-1842页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Ningbo Leader and Top Notch Person Training Project(No.20150012) 

主　　题：tumor necrosis factor-related apoptosisinducing ligand oridonin apoptosis choroidal melanoma 

摘      要：AIM:To investigate the inhibitory effect of the combined use of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)and oridonin on choroidal melanoma cell lines,and to explore its underlying ***:MUM-2B and C918 cells were treated with different concentrations of TRAIL and oridonin,and MTT assay used to evaluate the inhibition rate of the two compounds on ***,the cell cycle distribution and apoptosis were detected by flow cytometry,and changes in apoptosis-related proteins such as death receptor 5(DR5),a-caspase-3,and x-linked inhibitor of apoptosis protein(XIAP)were detected by Western ***-2B cells were transfected with si-DR5,which interfered with the expression of the DR5 *** and Western blot assay were used to detect cell activity and apoptosis-related ***:When TRAIL and oridonin were simultaneously administered to the MUM-2B cells,the apoptosis rate was significantly higher than that by the two drugs ***,the effect of combined use of TRAIL and oridonin on C918 cells was not significantly different from that used *** cycle analysis showed that TRAIL and oridonin could induce G2/M arrest in MUM-2B *** Western blot results showed that the protein expression levels of the DR5,a-caspase-3,and BAX increased,while the expression levels of the anti-apoptosis-related proteins XIAP and BCL-2 were suppressed when TRAIL and oridonin simultaneously administered to MUM-2B *** the expression of DR5 gene in MUM-2B cells could reverse the inhibitory effect of oridonin and TRAIL on the proliferation and apoptosis induction of MUM-2B ***:The inhibitory effects of oridonin and TRAIL on MUM-2B cells are significantly enhanced when they were administered as a combined treatment,which may ascribe to up-regulation of DR5.