Semen microbiota in normal and leukocytospermic males
作者机构：Health Management CenterThe Third Xiangya HospitalCentral South UniversityChangsha 410013China Institute of Integrated Traditional Chinese and Western MedicineXiangya HospitalCentral South UniversityChangsha 410005China Department of NephrologyIntegrated Hospital of Traditional Chinese and Western MedicineSouthern Medical UniversityGuangzhou 510220China Department of Traditional Chinese MedicineGuangdong Second Provincial General HospitalGuangzhou 510220China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2022年第24卷第4期
页 面：398-405页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：supported by Youth Foundation of National Natural Science Foundation of China(No.81703963 and No.81202807) the Natural Science Foundation of Hunan(No.2018JJ2667 and No.2019JJ40511) the Scientific Research Project of Chinese Traditional Medicine Administration Bureau in Hunan Province(No.201806)
主 题：16S rRNA gene sequencing asthenozoospermia leukocytospermia semen microbiota sperm motility
摘 要：Large numbers of microbes can be present in seminal fluid,and there are differences in the semen microbiota between normal and abnormal semen *** evaluate the semen microbiota in patients with leukocytospermia,87 seminal fluid samples,including 33 samples with a normal seminal leukocyte count and 54 samples with leukocytospermia,were obtained for a cross-sectional *** samples with a normal seminal leukocyte count had normal sperm parameters(Control group),and 13 samples with a normal seminal leukocyte count were from asthenozoospermia patients(Ast group).However,32 samples with leukocytospermia were from asthenozoospermia patients(LA group),and only 22 samples with leukocytospermia had normal sperm parameters(Leu group).The 16S ribosomal RNA(rRNA)gene sequencing method was used to sequence the microbiota in the seminal fluid,and multiple bioinformatics methods were utilized to analyze the ***,the results showed that the worse sperm parameters were observed in the leukocytospermia-related *** microbiota analysis found that there was increased alpha diversity in the leukocytospermia-related ***,Proteobacteria,Actinobacteria,and Bacteroidetes were the primary phyla in the seminal *** microbiota profiles,namely,Lactobacillus-enriched and Streptococcus-enriched groups,were identified in this *** majority of the samples in the groups with a normal seminal leukocyte count could be categorized as Lactobacillus-enriched,whereas the majority of the leukocytospermia samples could be categorized as *** study indicated that males with leukocytospermia have worse sperm parameters and a different semen microbiota composition compared to males with a normal seminal leukocyte count.
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