Anti-inflammatory and anti-apoptotic effects of N-acetylcysteine in diabetic rat corneal epithelium

作     者：Sae-Byeok Hwang Jin Hyoung Park Ji-Yun Park Soon-Suk Kang Ho Seok Chung Hun Lee Jae Yong Kim Hungwon Tchah 

作者机构：Research Institute of Miso Eye ClinicGyeonggi-do 13640Republic of Korea Biomedical Research CenterAsan Institute for Life ScienceAsan Medical CenterSeoul 05505Republic of Korea Miso Eye ClinicGyeonggi-do 13640Republic of Korea Department of OphthalmologyUniversity of Ulsan College of MedicineSeoul 05505Republic of Korea Research Institute for BiomacromoleculesUniversity of Ulsan College of MedicineSeoul 05505Republic of Korea Department of OphthalmologyDankook University HospitalDankook University College of MedicineCheonan 31116Republic of Korea 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2021年第14卷第12期

页      面：1805-1812页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：a Student Research Grant(2019)from the University of Ulsan College of Medicine Seoul Republic of Korea 

主　　题：N-acetylcysteine apoptosis inflammation diabetes corneal epithelium rat 

摘      要：AIM:To characterize the anti-inflammatory and antiapoptotic effects of N-acetylcysteine(NAC)in streptozotocin(STZ)-induced diabetic rat corneal epithelium and human corneal epithelial cells(HCECs)exposed to a high-glucose ***:HCECs were incubated in 0,5,50 mmol/L glucose medium,or 50 mmol/L glucose medium with NAC for *** was induced in rats by intraperitoneal injection of 65 mg/kg STZ and some of these rats were topically administered NAC to corneas with 3 mice per *** characterized receptor for advanced glycation end-products(RAGE)expression using immunofluorescence,and interleukin(IL)-1βand cleaved caspase-3(CCAP-3)expression using *** tumor necrosis factor(TNF)-αconcentration was measured by ELISA and cleaved poly-ADP ribose polymerase(PARP)concentration was quantified by Western *** cells were detected using TUNEL assay and annexin V and propidium iodide ***:Diabetic rats had higher expression of RAGE(2.46±0.13 fold),IL-1β,and CCAP-3 in apoptotic cells of their corneas than control *** expression of RAGE(1.83±0.11 fold),IL-1β,and CCAP-3,and the number of apoptotic cells,were reduced by topical NAC *** incubated in 50 mmol/L glucose medium showed high concentrations of TNF-α(310±2.00 pg/mL)and cleaved PARP(7.43±0.56 fold),and more extensive apoptosis than cells in 50 mmol/L glucose ***,the addition of NAC reduced the concentrations of TNF-α(153.67±2.31 pg/mL)and cleaved PARP(5.55±0.31 fold)and the number of apoptotic ***:NAC inhibits inflammation and apoptosis in the corneas of diabetic rats and HCECs maintained in a high-glucose environment.