XRCC1 Arg399Gln and Arg194Trp polymorphisms regulate XRCC1 expression and chemoresistance of non-small cell lung cancer cells

作     者：DAIRONG LI XIANLU ZHUO LUMI HUANG XIAOHUI JI DONGLIN WANG 

作者机构：Chongqing University Cancer Hospital&Chongqing Cancer Institute&Chongqing Cancer HospitalChongqing400030China Postdoctoral Scientific Research StationChongqing Cancer InstituteChongqing400030China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2019年第43卷第3期

页      面：139-144页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported in part by grants from the Chongqing Municipal Science and Technology Commission(#2013jcyjA10125). 

主　　题：Chemoresistance Non-small-cell lung cancer SNP XRCC1 

摘      要：X-ray repair cross-complementing protein 1(XRCC1)could repair cisplatin-induced DNA damage.XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function,leading to changes in cancer sensitivity to cisplatin treatment.This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability,apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP non-small cell lung cancer(NSCLC)cells.Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells.RT-PCR,Western blot,MTT assay,and flow cytometry analysis were performed to assess cell viability,apoptosis,and XRCC1 expression.Compared to control cells,the viability of A549 and A549/DDP cells transfected with XRCC1 Arg399Gln and Arg194Trp was higher and the apoptosis rate was lower,and XRCC1 mRNA and protein expression levels were significantly higher.In conclusion,our results suggest that XRCC1 Arg399Gln and Arg194Trp polymorphisms change XRCC1 expression in NSCLC cells and alter the sensitivity of NSCLC to cisplatin-based chemotherapy.