Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens

作     者：Pengsong Li Yumingzi Wang Xin Yuan Xinying Liu Chunmao Liu Xiaofen Fu Dezhi Sun Yan Dang Dawn E.Holmes 

作者机构：Beijing Key Laboratory for Source Control Technology of Water PollutionEngineering Research Center for Water Pollution Source Control and EcoremediationCollege of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Key Laboratory of Beijing Yanjing Beer Brewing TechnologyBeijing Yan Jing Brewery Company Ltd.Beijing 101300China MOST-USDA Joint Research Center for BiofuelsBeijing Engineering Research Center for BiofuelsInstitute of New Energy TechnologyTsinghua UniversityBeijing 100084China Department of Physical and Biological SciencesWestern New England University1215 Wilbraham RdSpringfieldMA 01119USA 

出 版 物：《Environmental Science and Ecotechnology》 (环境科学与生态技术（英文）)

年 卷 期：2021年第6卷第2期

页      面：3-10页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 0706[理学-大气科学] 0811[工学-控制科学与工程] 0713[理学-生态学] 

基　　金：supported by the Fundamental Research Funds for the Central Universities[grant numbers BLX201934,2019ZY19] Beijing Municipal Education Commission through Innovative Transdisciplinary Program“Ecological Restoration Engineering”. 

主　　题：Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon 

摘      要：In this study,an Escherichia coli(E.coli)whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein(GFP)reporter gene under the control of the ArsR1(GSU2952)regulatory circuit from Geobacter sulfurreducens.E.coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium.Under optimal test conditions,the Geobacter arsR1 promoter had a detection limit of 0.01 mM arsenite and the GFP expression was linear within a range of 0.03-0.1 mM(2.25-7.5 mg/l).These values were well below World Health Organization’s drinking water quality standard,which is 10 mg/l.The feasibility of using this whole-cell biosensor to detect arsenic in water samples,such as arsenic polluted tap water and landfill leachate was verified.The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry.This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.