Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation
作者机构：Henan Medical Key Laboratory of Emergency and Trauma ResearchThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Key Laboratory of Hepatobiliary and Pancreatic Surgery and Digestive Organ Transplantation of Henan ProvinceThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2021年第27卷第44期
页 面：7669-7686页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：Key Research Projects of Henan Higher Education Institutions (20A320064)
主 题：Severe acute pancreatitis Acute lung injury Calycosin Mouse model Highmobility group box 1 Nuclear factor-kappa B
摘 要：BACKGROUND Acute lung injury(ALI)is a common and life-threatening complication of severe acute pancreatitis(SAP).There are currently limited effective treatment options for SAP and associated ***(Cal),a bioactive constituent extracted from the medicinal herb Radix Astragali exhibits potent anti-inflammatory properties,but its effect on SAP and associated ALI has yet to be *** To identify the roles of Cal in SAP-ALI and the underlying *** SAP was induced via two intraperitoneal injections of L-arg(4 g/kg)and Cal(25 or 50 mg/kg)were injected 1 h prior to the first L-arg *** were sacrificed 72 h after the induction of SAP and associated ALI was examined histologically and *** in vitro model of lipopolysaccharide(LPS)-induced ALI was established using A549 *** analysis and western blot were evaluated in *** docking analyses were conducted to examine the interaction of Cal with *** Cal treatment substantially reduced the serum amylase levels and alleviated histopathological injury associated with SAP and *** infiltration and lung tissue levels of neutrophil mediator myeloperoxidase were reduced in line with protective effects of Cal against ALI in *** treatment also attenuated the serum levels and mRNA expression of pro-inflammatory cytokines tumor necrosis factor-α,interleukin-6,IL-1β,HMGB1 and chemokine(CXC motif)ligand 1 in lung *** and western blot analyses showed that Cal treatment markedly suppressed the expression of HMGB1 and phosphorylated nuclear factor-kappa B(NF-κB)p65 in lung tissues and an in vitro model of LPSinduced ALI in A549 cells suggesting a role for HGMB1 in the pathogenesis of ***,molecular docking analysis provided evidence for the direct interaction of Cal with *** Cal protects mice against L-arg-induced SAP and associated ALI by attenuating local and systemic neutrophil infiltration a
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