Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis

作     者：Lingzhi Jing Suna Fan Xiang Yao Yaopeng Zhang 

作者机构：State Key Laboratory for Modification of Chemical Fibers and Polymer MaterialsShanghai Engineering Research Center of Nano-Biomaterials and Regenerative MedicineCollege of Materials Science and EngineeringDonghua UniversityShanghai 201620P.R.China Jinan Jinquan Bio-Technology Co.LtdJinan 250101P.R.China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第6期

页      面：199-208页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by the National Key Research and Development Program of China(2018YFC1105800,2018YFC1105801) the Program of Shanghai Academic/Technology Research Leader(20XD1400100) the Natural Science Foundation of Shanghai(20ZR1402400) the National Natural Science Foundation of China(51703033,52173031,51903045) the Basic Research Project of the Science and Technology Commission of Shanghai Municipality(21JC1400100) the International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality(19520744500) the Fundamental Research Funds for the Central Universities(2232020D-04,2232019A3-06,2232019D3-02) 

主　　题：compound stimulation microfluidic chip stem cell cell proliferation cell differentiation 

摘      要：Bone tissue with strong adaptability is often in a complex dynamical microenvironment in vivo,which is associated with the pathogenesis and treatment of orthopedic ***,it is of great significance to investigate the effects of corresponding compound stimulation on cell ***,a fluid shear stress(FSS)plus ultrasound stimulation platform suitable for cell studies based on a microfluidic chip was constructed and bone marrow mesenchymal stem cell(BMSC)was chosen as a model *** proliferation and osteogenesis of BMSCs under the compound stimulation of FSS plus ultrasound in growth medium without any soluble induction factors were firstly *** FSS stimulation and static culture conditions were also *** illustrated that suitable single FSS stimulation(about 0.06 dyn/cm^(2))could significantly enhance cell proliferation and osteogenesis simultaneously when compared to the static control,while greater FSS mitigated or even restricted these enhancing ***,ultrasound stimulation combined with this suitable FSS stimulation further accelerated cell proliferation as the intensity of ultrasound *** for the osteogenesis under compound stimulation,it was relatively restricted under lower ultrasound intensity(about 0.075W/cm^(2)),while promoted when the intensity became higher(about 1.75W/cm^(2)).This study suggests that both the cell proliferation and osteogenesis are very responsive to the magnitudes of FSS and ultrasound stimulations and can be both significantly enhanced by proper combination ***,these findings will provide valuable references for the construction of effective cell bioreactors and also the treatment of orthopedic diseases.