Adolescent alcohol exposure alters DNA and RNA modifications in peripheral blood by liquid chromatography-tandem mass spectrometry analysis

作     者：Meng-Yuan Chen Zhu Gui Ke-Ke Chen Jiang-Hui Ding Jin-Gang He Jun Xiong Jia-Le Li Jie Wang Bi-Feng Yuan Yu-Qi Feng Meng-Yuan Chen;Zhu Gui;Ke-Ke Chen;Jiang-Hui Ding;Jin-Gang He;Jun Xiong;Jia-Le Li;Jie Wang;Bi-Feng Yuan;Yu-Qi Feng

作者机构：Sauvage Center for Molecular SciencesDepartment of ChemistryWuhan UniversityWuhan 430072China Key Laboratory of Magnetic Resonance in Biological SystemsState Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsWuhan 430071China School of Health SciencesWuhan UniversityWuhan 430071China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第4期

页      面：2086-2090页

核心收录：

学科分类：1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 100205[医学-精神病与精神卫生学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (Nos. 22074110, 21635006, 21721005, 31771193) the Fundamental Research Funds for the Central Universities (No.2042021kf0212) 

主　　题：Alcohol exposure Adolescence Peripheral blood DNA modification RNA modification Mass spectrometry 

摘      要：Alcohol consumption is a critical risk factor contributing to a verity of human diseases. The incidence of alcohol use disorder increases across adolescence in recent years. Accumulating line of evidence suggests that alcohol-induced changes of DNA cytosine methylation(5-methyl-2-deoxycytidine, 5 m C) in genomes play an important role in the development of diseases. However, systemic investigation of the effects of adolescent alcohol exposure on DNA and RNA modifications is still lacked. Especially, there hasn’t been any report to study the effects of alcohol exposure on RNA modifications. Similar to DNA modifications,RNA modifications recently have been identified to function as new regulators in modulating numbers of biological processes. In the current study, we systematically investigated the effects of alcohol exposure on both DNA and RNA modifications in peripheral blood of adolescent rats by liquid chromatographyelectrospray ionization-tandem mass spectrometry(LC-ESI-MS/MS) analysis. The developed LC-ESI-MS/MS method enabled the sensitive and accurate determination of 2 DNA modifications and 12 RNA modifications. As for the alcohol exposure experiments, the adolescent rats were intraperitoneally injected with ethanol with an interval of one day for a total 14 days. The quantification results by LC-ESI-MS/MS analysis showed that adolescent alcohol exposure could alter both DNA and RNA modifications in peripheral blood. Specifically, we observed an overall decreased trend of RNA modifications. The discovery of the significant alteration of the levels of DNA and RNA modifications under alcohol exposure indicates that alcohol consumption may increase the risk of the incidence and development of diseases through dysregulating DNA and RNA modifications.