PoreScript:Semi-automated pore size algorithm for scaffold characterization

作     者：Dana Jenkins Karim Salhadar Grant Ashby Anita Mishra Joy Cheshire Felipe Beltran Melissa Grunlan Sebastien Andrieux Cosima Stubenrauch Elizabeth Cosgriff-Hernandez 

作者机构：Department of Biomedical EngineeringUniversity of Texas–AustinAustinTX78712USA Department of Biomedical EngineeringTexas A&M UniversityCollege StationTX77843USA Universite de StrasbourgCNRSInstitut Charles Sadron UPR22F-67000StrasbourgFrance Institut für Physikalische ChemieUniversitat StuttgartPfaffenwaldring 55D-70569StuttgartGermany 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2022年第7卷第7期

页      面：1-8页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 081203[工学-计算机应用技术] 08[工学] 0835[工学-软件工程] 0836[工学-生物工程] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：The authors would like to acknowledge the following funding support:National Institutes of Health[NIH-R21 AR076708] National Science Foundation[NSF-BSF 1822196,DGE-1610403] 

主　　题：Pore size measurement Polymer scaffolds MATLAB program Tissue engineering 

摘      要：The scaffold pore size influences many critical physical aspects of tissue engineering,including tissue infiltration,biodegradation rate,and mechanical *** measurements of pore sizes from scanning electron micrographs using ImageJ/FIJI are commonly used to characterize scaffolds,but these methods are both time-consuming and subject to user *** semi-automated analysis tools are limited by a lack of accessibility or limited sample size in their verification *** work here describes the development of a new MATLAB algorithm,PoreScript,to address these *** algorithm was verified using three common scaffold fabrication methods(e.g.,salt leaching,gas foaming,emulsion templating)with varying pore sizes and shapes to demonstrate the versatility of this new *** results demonstrate that the pore size characterization using PoreScript is comparable to manual pore size *** PoreScript algorithm was further evaluated to determine the effect of user-input and image parameters(relative image magnification,pixel intensity threshold,and pore structure).Overall,this work validates the accuracy of the PoreScript algorithm across several fabrication methods and provides user-guidance for semi-automated image analysis and increased throughput of scaffold characterization.