Overexpression of miR-101 suppresses collagen synthesis by targeting EZH2 in hypertrophic scar fibroblasts

作     者：Jie Li Yan Li Yunchuan Wang Xiang He Jing Wang Weixia Cai Yanhui Jia Dan Xiao Jian Zhang Ming Zhao Kuo Shen Zichao Li Wenbin Jia Kejia Wang Yue Zhang Linlin Su Huayu Zhu Dahai Hu 

作者机构：Department of Burns and Cutaneous SurgeryXijing HospitalFourth Military Medical UniversityXi’anShaanxi710032China 

出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)

年 卷 期：2021年第9卷第1期

页      面：83-94页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(81772071 to DHH) 

主　　题：Hypertrophic scars miR-101 EZH2 Collagen Fibroblasts Skin Collagen 

摘      要：Background:MicroRNA-101(miR-101)is a tumor suppressor microRNA(miRNA)and its loss is associated with the occurrence and progression of various ***,the biological function and target of miR-101 in the pathogenesis of hypertrophic scars(HS)remains ***:We harvested HS and paired normal skin(NS)tissue samples from patients and cultured their fibroblasts(HSF and NSF,respectively).We used quantitative reverse transcriptase polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),enzyme-linked immunosorbent assays(ELISA)and Western blot analyses to measure mRNA levels and protein expression of miR-101,enhancer of zeste homolog 2(EZH2),collagen 1 and 3(Col1 and Col3)andα-smooth muscle actin(α-SMA)in different in vitro *** also used RNA sequencing to evaluate the relevant signaling pathways and bioinformatics analysis and dual-luciferase reporter assays to predict miR-101 *** utilized a bleomycin-induced fibrosis mouse model in which we injected miR-101 mimics to evaluate collagen deposition in ***:We found low expression of miR-101 in HS and HSF compared to NS and *** miR-101 decreased Col1,Col3 andα-SMA expression in *** detected high expression of EZH2 in HS and *** of EZH2 decreased Col1,Col3 andα-SMA in ***,miR-101 targeted the 3-untranslated region(3UTR)of EZH2,as indicated by the decreased expression of *** EZH2 rescued miR-101-induced collagen ***-101 mimics effectively suppressed collagen deposition in the bleomycin-induced fibrosis mouse ***:Our data reveal that miR-101 targets EZH2 in HS collagen production,providing new insight into the pathological mechanisms underlying HS formation.