Multiplexed Profiling of Extracellular Vesicles for Biomarker Development

作     者：Cheng Jiang Ying Fu Guozhen Liu Bowen Shu Jason Davis George K.Tofaris Cheng Jiang;Ying Fu;Guozhen Liu;Bowen Shu;Jason Davise;George K.Tofaris

作者机构：Nuffield Department of Clinical NeurosciencesNew Biochemistry BuildingUniversity of OxfordOxford OX13QUUK Department of ChemistryUniversity of OxfordOxford OX13QZUK School of Life and Health SciencesThe Chinese University of Hong KongShenzhen 518172People’s Republic of China Dermatology HospitalSouthern Medical UniversityGuangzhou 510091People’s Republic of China Kavli Institute for Nanoscience DiscoveryNew Biochemistry BuildingUniversity of OxfordOxfordUK 

出 版 物：《Nano-Micro Letters》 (纳微快报（英文版）)

年 卷 期：2022年第14卷第1期

页      面：59-94页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：funded by grants from the EPSRC(EP/M006204/1) the Michael J Fox Foundation the Selfridges Group Foundation the NIHR Oxford Biomedical Research Centre to G.K.T and J.J.D support from the John Fell Fund(HMD00470) 

主　　题：Multiplexed profiling Extracellular vesicles Exosomes Liquid biopsy Point-of-care Biomarker 

摘      要：Extracellular vesicles(EVs)are cell-derived membranous particles that play a crucial role in molecular trafficking,intercellular transport and the egress of unwanted *** have been implicated in many diseases including cancer and *** are detected in all bodily fluids,and their protein and nucleic acid content offers a means of assessing the status of the cells from which they *** such,they provide opportunities in biomarker discovery for diagnosis,prognosis or the stratification of diseases as well as an objective monitoring of *** simultaneous assaying of multiple EV-derived markers will be required for an impactful practical application,and multiplexing platforms have evolved with the potential to achieve ***,we provide a comprehensive overview of the currently available multiplexing platforms for EV analysis,with a primary focus on miniaturized and integrated devices that offer potential step changes in analytical power,throughput and consistency.