Protective effect and mechanism of dexmedetomidine on lung injury in diabetic mice with myocardial ischemia reperfusion
作者机构：Department of AnesthesiologyThe First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054China
出 版 物：《Journal of Hainan Medical University》 (海南医学院学报（英文版）)
年 卷 期：2021年第27卷第19期
页 面：15-20页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2019D01C298)
主 题：Dexmedetomidine Sirt1 Endoplasmic reticulum stress Lung injury
摘 要：Objective:To evaluate the protective effect of dexmedetomidine and its mechanism on the lung after myocardial ischemia reperfusion in diabetic ***:Adult diabetic db/db mice aged 15 weeks were *** sham operation group(S group),ischemic reperfusion group(IR group),dexmedetomidine post-treatment group(POST-D)and dexmedetomidine pre-treatment group(PRE-D)were set *** IR group of mice underwent ligation of anterior descending branch of left coronary artery for 30min and readministration for *** post-D group and the pre-D group were intraperitoneally injected with 50 ug/kg DEX before surgery and during reperfusion,*** blood was extracted at 120min of reperfusion,serum levels of creatine kinase isoenzyme MB and inflammatory cytokines IL-6,IFN-γand IL-10 were detected,then mice were sacrificed and lung tissue was taken,the ratio of wet and dry weight(W/D)was determined,lung histopathological morphology was observed under light microscope,superoxide dismutase(SOD)and malondialdehyde(MDA)contents were determined,Western Blot was used to detect the expressions of Sirt1,GRP78 and CHOP in lung ***:Compared with the S group,the IR group rats had severe lung injury,including increased lung W/D value,increased serum CK-MB、IL-6 and IFN-γlevels,decreased serum IL-10,increased serum MDA content,decreased SOD activity,down-regulated Sirt1 expression,up-regulated GRP78 and CHOP *** with IR group,lung histopathological injury was reduced in post-D group and pre-D group,lung W/D value was decreased,serum CK-MB、IL-6 and IFN-γlevels were decreased,serum IL-10 was increased,serum MDA content was reduced,SOD activity was rised,the expression of Sirt1 was up-regulated,and GRP78 and CHOP were *** with the post-D group,the degree of lung tissue injury in the pre-D group was reduced,but the differences in various indicators were not statistically ***:Dexmedetomidine can allevia
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