Fusobacterium nucleatum colonization is associated with decreased survival of helicobacter pylori-positive gastric cancer patients
作者机构：Department of Gastroenterology and HepatologyChiayi Chang Gung Memorial HospitalChiayi 61301Taiwan College of MedicineChang Gung UniversityTaoyuan 33302Taiwan Department of Applied MathematicsNational Chiayi UniversityChiayi 60035Taiwan Department of Biomedical SciencesNational Chung Cheng UniversityChiayi 62130Taiwan
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2021年第27卷第42期
页 面：7311-7323页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：by Chiayi Chang Gung Memorial Hospital grants,including No.CORPG6G0021,No.CORPG6G0022 and No.CORPG6G0023 to Wu CS No.CORPG6G0061,No.CORPG6G0062 and No.CORPG6G0063 to Tung SY No.CORPG6G0071,No.CORPG6G0072 and No.CORPG6G0073 to Hsieh YY
主 题：Fusobacterium nucleatum Helicobacter pylori Gastric cancer Survival Interferon Mobility
摘 要：BACKGROUND An increased amount of Fusobacterium nucleatum(***)is frequently detected in the gastric cancer-associated microbiota of the Taiwan Residents *** is known to exert cytotoxic effects and play a role in the progression of colorectal cancer,though the impact of *** colonization on gastric cancer cells and patient prognosis has not yet been *** To identify ***-dependent molecular pathways in gastric cancer cells and to determine the impact of *** on survival in gastric *** Coculture of *** with a gastric cancer cell line was performed,and changes in gene expression were *** with significant changes in expression were identified by RNA *** analysis was carried out to determine deregulated cellular functions.A cohort of gastric cancer patients undergoing gastrectomy was recruited,and nested polymerase chain reaction was performed to detect the presence of *** in resected cancer *** analysis was performed to determine whether *** colonization affects patient *** RNA sequencing and subsequent pathway analysis revealed a drastic interferon response induced by a high colonization *** response peaked within 24 h and subsided after 72 h of *** contrast,deregulation of actin and its regulators was observed during prolonged incubation under a low colonization load,likely altering the mobility of gastric cancer *** to the clinical specimen analysis,approximately one-third of the gastric cancer patients were positive for ***,and statistical analysis indicated that the risk for colonization increases in late-stage cancer *** analysis demonstrated that *** colonization was associated with poorer outcomes among patients also positive for Helicobacter pylori(***).CONCLUSION *** colonization leads to deregulation of actin dynamics and likely changes cance
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