TGF-β2-induced NEAT1 regulates lens epithelial cell proliferation,migration and EMT by the miR-26a-5p/FANCE axis

作     者：Xiao-Hui Yu Shao-Yi Liu Cheng-Fang Li 

作者机构：Department of Ophthalmologythe Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityYantai 264000Shandong ProvinceChina Department of OphthalmologyQingdao Hospital of Traditional Chinese Medicine(Qingdao Hiser Hospital)Qingdao 266033Shandong ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2021年第14卷第11期

页      面：1674-1682页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

主　　题：posterior capsule opacification transforming growth factor-beta 2 nuclear paraspeckle assembly transcript 1 miRNA-26a-5p fanconi anemia complementation group E 

摘      要：AIM:To explore the regulatory mechanism of nuclear paraspeckle assembly transcript 1(NEAT1)in the pathogenesis of posterior capsule opacification(PCO).METHODS:Quantitative reverse transcription polymerase chain reaction(RT-q PCR)was executed to analyze NEAT1 and micro RNA(miR)-26a-5p expression in transforming growth factor-beta 2(TGF-β2)-disposed lens epithelial cells(LECs).The proliferation,cell cycle progression,apoptosis,and migration of TGF-β2-disposed LECs were *** relationship between NEAT1 or fanconi anemia(FA)complementation group E(FANCE)and miR-26a-5p was verified by dual-luciferase reporter ***:TGF-β2 induced NEAT1 expression in ***1 inhibition accelerated apoptosis,cell cycle arrest,decreased proliferation,epithelial-mesenchymal transition(EMT),and migration of TGF-β2-disposed ***1 sponged miR-26a-5p to further regulate FANCE *** experiments presented that miR-26a-5p downregulation overturned NEAT1 silencing-mediated impacts on TGF-β2-disposed LEC biological ***,FANCE overexpression reversed miR-26a-5p mimic-mediated impacts on TGF-β2-disposed LEC biological ***:TGF-β2-induced NEAT1 facilitates LEC proliferation,migration,and EMT by upregulating FANCE via sequestering miR-26a-5p.