Efficacy of the herbal pair, Radix Achyranthis Bidentatae and Eucommiae Cortex, in preventing glucocorticoid-induced osteoporosis in the zebrafish model

作     者：Joon-ho Lee Yuan-ji Wei Zhong-yan Zhou Yu-ming Hou Cheng-long Wang Li-bo Wang Hong-jin Wu Yu Zhang Wei-wei Dai Joon-ho Lee;Yuan-ji Wei;Zhong-yan Zhou;Yu-ming Hou;Cheng-long Wang;Li-bo Wang;Hong-jin Wu;Yu Zhang;Wei-wei Dai

作者机构：Central Laboratory of Science and Technology DepartmentLonghua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Cardiovascular DepartmentLonghua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2022年第20卷第1期

页      面：83-90页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：funded by the Natural Science Foundation of China(No.81873318) the Program Foundation of Shanghai Health and Family Planning Commission,China(No.2018JP013) the Ph.D.Program Foundation of Shanghai Municipal Education Commission,China(SMEC,No.B201713) the Postgraduate Innovation Program of Shanghai University of TCM(2020JX357) 

主　　题：Glucocorticoid Osteoporosis Radix Achyranthis Bidentatae Eucommiae Cortex Zebrafish 

摘      要：Objective: In traditional Chinese medicine, the herbal pair, Radix Achyranthis Bidentatae(RAB) and Eucommiae Cortex(EC), is widely used to treat osteoporosis. Herein, we determined whether this herbal pair can be used to ameliorate glucocorticoid(GC)-induced osteoporosis(GIOP) and find its optimal dosage in ***: The characteristics of the aqueous extract of RAB and EC were separately characterized using high-performance liquid chromatography. Osteoporosis was induced in 5-day post-fertilization zebrafish larvae by exposing them to 10 lmol/L dexamethasone(Dex) for 96 h. Seven combinations of different ratios of RAB and EC were co-administered. Treatment efficacy was determined by calculating zebrafish vertebral area and sum brightness, via alizarin red staining, and by detecting alkaline phosphatase(ALP)activity. Multiple regression analysis was conducted to test the optimal dosage ***: According to the Chinese Pharmacopoeia(2015), b-ecdysone(b-Ecd) is a major bioactive marker in RAB extract, while pinoresinol diglucoside(PDG) is the major marker in EC extract. Both of b-Ecd and PDG content values aligned with the Chinese Pharmacopoeia standards. Treatment with 10 lmol/L Dex reduced zebrafish vertebral area, sum brightness, and ALP activity, but RAB and EC attenuated these effects. Combining 50 mg/m L RAB and 50 mg/m L EC was optimal for preventing GIOP in *** transcription-quantitative polymerase chain reaction was used to evaluate the m RNA expression of osteogenesis-related genes. A treatment of 10 lmol/L Dex decreased runt-related transcription factor 2(Runx2), osteogenic protein-1(OP-1), bone c-carboxyglutamic acid-containing protein(BGLAP), and bcatenin levels. This effect was counteracted by RAB and EC co-treatment(P 0.05). Additionally, the effect of using the two herbal extracts together was better than single-herb treatments separately. These results demonstrated that RAB and EC preserve osteoblast function in the pre