Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease

作     者：Jia-Nan Yan Hai-Ying Zhang Jun-Rui Li Ying Chen Yong-Cheng Jiang Jia-Bing Shen Kai-Fu Ke Xiao-Su Gu Jia-Nan Yan;Hai-Ying Zhang;Jun-Rui Li;Ying Chen;Yong-Cheng Jiang;Jia-Bing Shen;Kai-Fu Ke;Xiao-Su Gu

作者机构：Department of NeurologyAffiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Research Center of Clinical MedicineAffiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Clinical MedicineThe First Clinical Medical College of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Neurology and Suzhou Clinical Research Center of Neurological DiseaseThe Second Afflicted Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2022年第17卷第6期

页      面：1357-1363页

核心收录：

学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：Technology Project of Nantong of China,Nos.JC2020052(to XSG),JCZ19087(to XSG) the National Natural Science Foundation of China,Nos.81873742(to KFK),81901195(to JBS),81502867(to TX),82073627(to TX). 

主　　题：alpha-synuclein autophagosomes autophagy neural regeneration neuroprotection Parkinson’s disease PI3K/AKT/mTOR pathway skin-derived precursor Schwann cells 

摘      要：Autophagy has been shown to play an important role in Parkinson’s disease.We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson’s disease through affecting autophagy.In this study,6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells(SKP-SCs).The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine,reduced excessive autophagy,increased tyrosine hydroxylase expression,reducedα-synuclein expression,reduced the autophagosome number,and activated the PI3K/AKT/mTOR pathway.Autophagy activator rapamycin inhibited the effects of SKP-SCs,and autophagy inhibitor 3-methyladenine had the opposite effect.These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy,thereby exhibiting a neuroprotective effect in a cellular model of Parkinson’s disease.This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University(approval No.S20181009-205)on October 9,2018.