The anti-neoplastic activities of aloperine in HeLa cervical cancer cells are associated with inhibition of the IL-6-JAK1-STAT3 feedback loop

作     者：CHEN Yao-Dong CAI Fang-Yu MAO Yu-Ze YANG Yong-Sheng XU Kun LIU Xiao-Fang FAN Wen-Wen CHEN Wu JIANG Feng-Qi ZHANG Hui CHEN Yao-Dong;CAI Fang-Yu;MAO Yu-Ze;YANG Yong-Sheng;XU Kun;LIU Xiao-Fang;FAN Wen-Wen;CHEN Wu;JIANG Feng-Qi;ZHANG Hui

作者机构：Department of Ultrasonic Imagingthe First Hospital of Shanxi Medical UniversityTaiyuan 030001China Department of Radiologythe First Clinical Medical CollegeShanxi Medical UniversityTaiyuan 030001China Department of Thoracic Surgerythe General Hospital of Heilongjiang Province Land Reclamation BureauHarbin 150088China Department of Cardio-Thoracic Surgerythe First Affiliated Hospital of Jiamusi UniversityJiamusi 154000China Department of General SurgeryHeilongjiang Provincial HospitalHarbin 150001China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2021年第19卷第11期

页      面：815-824页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China(No.82001850) Shanxi Basic Application Research(No.201901D211491) the Scientific Research Project of Shanxi Health Commission(No.2019038) the Doctoral Research Project of Shanxi Medical University(No.XD1901) the Doctoral Research Project of Shanxi Province(No.SD1901) 

主　　题：Cervical cancer Chemotherapy Aloperine Epithelial-mesenchymal transition IL-6-JAK1-STAT3 feedback loop 

摘      要：Cervical cancer(CC)is recognized as the most common neoplasm in the female reproductive system *** lack of chemotherapeutic agents with outstanding effectiveness and safety severely compromises the anti-cipated prognosis of ***(ALO)is a natural quinolizidine alkaloid with marked anti-cancer effects on multiple malignancies as well as favorable activity in relieving inflammation,allergies and ***,its therapeutic efficacy and underlying mechanism in CC are still *** the current study,MTT assay was employed to evaluate the viability of HeLa cells exposed to ALO to preliminarily estimate the effectiveness of ALO in ***,the effects of ALO on the proliferation and apoptosis of HeLa cells were further investigated by plate colony formation and flow cytometry,respectively,while the migration and invasion of ALO-treated HeLa cells were evaluated using Transwell ***,nude mice were subcutaneously inoculated with HeLa cells to demonstrate the anti-CC properties of ALO in *** molecular mechanisms underlying these effects of ALO were evaluated by Western blot and immunohistochemical *** study experimentally demonstrated that ALO inhibited the proliferation of HeLa cells via G2 phase cell cycle ***,ALO promoted an increase in the percentage of apoptotic HeLa cells by increasing the Bax/Bcl-2 ***,the migration and invasion of HeLa cells were attenuated by ALO treatment,which was considered to result from inhibition of epithelial-to-mesenchymal *** molecular mechanisms,the expression and activation of the IL-6-JAK1-STAT3 feedback loop were markedly suppressed by ALO *** study indicated that ALO markedly suppresses the proliferation,migration and invasion and enhances the apoptosis of HeLa *** addition,these prominent anti-CC properties of ALO are associated with repression of the IL-6-JAK1-STAT3 feedback loop.