Cloning of catalase gene and antioxidant genes in Scophthalmus maximus response to metalloprotease of Vibrio anguillarum stress

作     者：Hai REN Jian LI Ping LIU Xianyun REN Tao SONG Guisheng GAO Duwen LI Shuaiting LIU Hai REN;Jian LI;Ping LIU;Xianyun REN;Tao SONG;Guisheng GAO;Duwen LI;Shuaiting LIU

作者机构：Hebei Key Laboratory of Preventive Veterinary MedicineHebei Normal University of Science and TechnologyQinhuangdao 066004China Key Laboratory of Sustainable Development of Marine FisheriesMinistry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2022年第40卷第1期

页      面：322-335页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：Supported by the National Natural Science Foundation of China(No.31502187) the Natural Science Foundation of Hebei Province(No.C2018407049) the Hebei Provincial Department of Science and Technology(Nos.20286701Z,20567621H) the Talent Engineering Training Funding Project of Hebei Province(No.A201901057) 

主　　题：gene cloning expression reactive oxygen species metalloprotease head kidney cells 

摘      要：Metalloproteases represent a class of extracellular proteases found in Vibrio anguillarum that can generate toxic and pathogenic eff ects in turbot(Scophthalmus maximus).The toxicological eff ect partly results from oxidative damage due to the production of excessive reactive oxygen species(ROS).Catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPx)are major antioxidant enzymes induced by various oxidative stresses and can scavenge peroxides generated in *** evaluate the eff ects of metalloprotease-induced ROS on the antioxidation defense mechanism of *** head kidney cells,the cDNA of CAT gene(designated as SmCAT)was cloned and *** comprises a 1584-bp coding sequence that encodes a protein containing 527 amino acids with a poly(A)*** analysis revealed an active site signature sequence,a heme-ligand signature sequence,and three catalytic amino acid *** deduced SmCAT amino acid sequence shares a sequence similarity of 66.1%-92.4%with those of other *** analysis revealed that SmCAT is classifi ed with CAT of other fi *** real-time PCR analysis showed that SmCAT was extensively expressed in all tested tissues,especially in *** expression of SmCAT,SmMnSOD,and SmGPx were inhibited signifi cantly in head kidney cells treated with metalloprotease from 12 to 24 *** 6 to 24 h metalloprotease-treated groups compared to that of the untreated group,it was found that the production of ROS was markedly increased,and the mitochondrial membrane potential was decreased *** 33342 staining revealed the presence of apoptotic bodies when the cells were incubated with 8.0 or 40.0μg/mL metalloprotease for 12 and 24 ***,the toxic eff ects of metalloprotease are associated with the down-regulation of antioxidant enzyme expression and increased ROS levels,which trigger the activation of apoptosis in the head kidney cells of *** fi ndings provide a better under