The high diversity of SARS-CoV-2-related coronaviruses in pangolins alerts potential ecological risks

作     者：Min-Sheng Peng Jian-Bo Li Zheng-Fei Cai Hang Liu Xiaolu Tang Ruochen Ying Jia-Nan Zhang Jia-Jun Tao Ting-Ting Yin Tao Zhang Jing-Yang Hu Ru-Nian Wu Zhong-Yin Zhou Zhi-Gang Zhang Li Yu Yong-Gang Yao Zheng-Li Shi Xue-Mei Lu Jian Lu Ya-Ping Zhang Min-Sheng Peng;Jian-Bo Li;Zheng-Fei Cai;Hang Liu;Xiaolu Tang;Ruochen Ying;Jia-Nan Zhang;Jia-Jun Tao;Ting-Ting Yin;Tao Zhang;Jing-Yang Hu;Ru-Nian Wu;Zhong-Yin Zhou;Zhi-Gang Zhang;Li Yu;Yong-Gang Yao;Zheng-Li Shi;Xue-Mei LU;Jian Lu;Ya-Ping Zhang

作者机构：State Key Laboratory of Genetic Resources and EvolutionYunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China Kunming College of Life ScienceUniversity of Chinese Academy of SciencesKunmingYunnan 650204China KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650201China State Key Laboratory for Conservation and Utilization of Bio-resources in YunnanYunnan UniversityKunmingYunnan 650091China State Key Laboratory of Protein and Plant Gene ResearchCenter for BioinformaticsSchool of Life SciencesPeking UniversityBeijing 100871China Molbreeding Biotechnology Co.Ltd.ShijiazhuangHebei 050035China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan ProvinceKunming Institute of ZoologyKunmingYunnan 650201China CAS Key Laboratory of Special Pathogens and BiosafetyWuhan Institute of VirologyChinese Academy of SciencesWuhanHubei 430071China Center for Excellence in Animal Evolution and GeneticsChinese Academy of SciencesKunmingYunnan 650201China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2021年第42卷第6期

页      面：834-844页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：This work was supported by the National Key Research and Development Projects of the Ministry of Science and Technology of China,National Key Research and Development Program of China(2021YFC0863300) Ministry of Agriculture of China(2016ZX08009003-006) Key Program of Chinese Academy of Sciences(KJZD-SW-L11) Animal Branch of the Germplasm Bank of Wild Species,Chinese Academy of Sciences(the Large Research Infrastructure Funding) 

主　　题：SARS-CoV-2 Pangolin Recombination Diversity Sequencing mtDNA 

摘      要：Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks.A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses(SC2r-CoVs).Here,we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species,and detected positive signals in muscles of four Manis javanica and,for the first time,one *** analysis of pangolin mitochondrial DNA traced their origins from Southeast *** in-solution hybridization capture sequencing,we assembled a partial pangolin SC2r-CoV(pangolin-CoV)genome sequence of 22895 bp(MP20)from the *** *** analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in *** seized by Guangxi Customs.A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was *** analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously *** the potential infectivity of pangolin-CoVs,the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs.