Transcriptional response of porcine skeletal muscle to feeding a linseed-enriched diet to growing pigs

作     者：Hongkui Wei Yuanfei Zhou Shuzhong Jiang Feiruo Huang Jian Peng Siwen Jiang 

作者机构：Department of Animal Nutrition and Feed Science College of Animal Science and Technology Huazhong Agricultural University Key Lab of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture Huazhong Agricultural University 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2016年第7卷第2期

页      面：238-247页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：National Program on Key Basic research Project (No.2012CB127305) Hubei Provincial Creative Team Project of Agricultural Science and Technology (No.2007-620) Hubei Provincial Natural Science Foundation (No.2013CFA010) Fundamental Research Funds for the Central Universities (2013PY047 and 2013QC004) 

主　　题：Linseed m RNA expression n 3 PUFAs Skeletal muscle 

摘      要：Background： To investigate the effect of feeding a linseed-enriched diet to growing-finishing pigs on gene expression in skeletal muscle, pigs were fed with a linseed-enriched diet for 0, 30, 60 and 90 d. Transcriptional profiles of longissimus dorsi muscle were measured using Affymetrix ***： Results showed that 264 genes were identified as differentially expressed genes（DEGs）. The strongest transcriptional response was clearly observed at 30 d. DEGs were assigned to several main functional terms,including transcription, apoptosis, intracellular receptor-mediated signaling, muscle organ development, fatty acid metabolic process, cell motion, regulation of glucose metabolic process, spermatogenesis and regulation of myeloid cell differentiation. We also found that transcriptional changs of several transcription cofactors might contribute to n-3 PUFAs regulated gene expression. In addition, the increased expression of IGF-1, insulin signaling pathway and the metabolism of amino acids might involve in the muscle growth induced by feeding a linseedenriched diet. The results also provide the new evidence that the expression changes of PTPN1, HK2 and PGC-1αmight contribute to the regulation of insulin sensitivity by n-3 ***： Our finding provided correlative evidence that feeding the linseed enriched diet affact expression of genes involved in insulin signaling pathway and the metabolism of amino acids.