Phosphorylation of MtRopGEF2 by LYK3 mediates MtROP activity to regulate rhizobial infection in Medicago truncatula

作     者：Mingxing Wang Huan Feng Peng Xu Qiujin Xie Jinpeng Gao Yanzhang Wang Xiaowei Zhang Jun Yang Jeremy DMurray Fengli Sun Chunyan Wang Ertao Wang Nan Yu Mingxing Wang;Huan Feng;Peng Xu;Qiujin Xie;Jinpeng Gao;Yanzhang Wang;Xiaowei Zhang;Jun Yang;Jeremy D.Murray;Fengli Sun;Chunyan Wang;Ertao Wang;Nan Yu

作者机构：College of Life and Environment SciencesShanghai Normal UniversityShanghai 200234China National key Laboratory of Plant Molecular GeneticsCenter for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and Ecologythe Chinese Academy of SciencesShanghai 200032China University of Chinese Academy of SciencesBeijing 100039China College of ForestryNorthwest A&F UniversityYangling 712100China College of AgronomyNorthwest A&F UniversityYangling 712100China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第10期

页      面：1787-1800页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：This work was supported by the National Key Research and Development Program of China(2016YFA0500502) the National Science Foundation(31825003,31730103,31870218) the Strategic Priority Research Program"Molecular Mechanism of Plant Growth and Development"of the Chinese Academy of Sciences(XDB27040207) CAS Project for Young Scientists in Basic Research(YSBR-011). 

主　　题：guanine nucleotide exchange factor ROP GTPase root hair deformation rhizobial infection 

摘      要：The formation of nitrogen-fixing no dules on legume roots requires the coordination of infection by rhizobia at the root epidermis with the initiation of cell divisions in the root cortex.During infection,rhizobia attach to the tip of elongating root hairs which then curl to entrap the rhizobia.However,the mechanism of root hair deformation and curling in response to symbiotic signals is still elusive.Here,we found that small GTPases(MtRac1/MtROP9 and its homologs)are required for root hair development and rhizobial infection in Medicago truncatula.Our results show that the Nod factor receptor LYK3 phosphorylates the guanine nucleotide exchange factor MtRopGEF2 at S73 which is critical for the polar growth of root hairs.In turn,phosphorylated MtRopGEF2 can activate MtRac1.Activated MtRac1 was found to localize at the tips of root hairs and to strongly interact with LYK3 and NFP.Taken together,our results support the hypothesis that MtRac1,LYK3,and NFP form a polarly localized receptor complex that regulates root hair deformation during rhizobial infection.