Proteomic identification of ruminal epithelial protein expression profiles in response to starter feed supplementation in pre-weaned lambs

作     者：Daming Sun Shengyong Mao Weiyun Zhu Junhua Liu Daming Sun;Shengyong Mao;Weiyun Zhu;Junhua Liu

作者机构：Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthLaboratory of Gastrointestinal MicrobiologyCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095Jiangsu ProvinceChina National Center for International Research on Animal Gut NutritionNanjing Agricultural UniversityNanjing 210095China National Experimental Teaching Demonstration Center of Animal ScienceNanjing Agricultural UniversityNanjing 210095China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第4期

页      面：1271-1282页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31501980) National Key Research and Development Plan(2018YFD0501900) project for top young talents program of College of Animal Science and Technology of Nanjing Agricultural University(DKQB201904) Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX20₀603) 

主　　题：Starter Rumen epithelium Protein expression Proteomics Lamb 

摘      要：The pre sent study aimed to comparatively characterize the ruminal epithelial protein expression profiles in lambs fed ewe milk or milk plus starter diet using proteome *** new-born lambs were randomly divided into a group receiving ewe milk(M,n=10)and a group receiving milk plus starter diet(M+S,n=10).From 10 d old,M group lambs remained with the ewe and suckled ewe milk without receiving the starter *** lambs in the M+S group were separated from the ewe and received starter *** lambs were slaughtered at 56 d *** rumen epithelia samples(4 per group)were collected to characterize their protein expression profiles using proteomic *** analysis showed that 31 upregulated proteins and 40 downregulated proteins were identified in the rumen epithelium of lambs in response to starter diet *** results showed that starter feeding regulates a variety of biological processes in the epithelium,especially blood vessel development and extracellular matrix protein ***,the expression of proteins associated with synthesis and degradation of ketone bodies,butanoate metabolism,and citrate cycle signaling transduction pathway were upregulated in the group with starter diet supplementation,including 3-hydroxy-3-methylglutaryl coenzyme A synthase(HMGCS2,fold change[FC]=1.93),3-hydroxybutyrate dehydrogenase 1(BDH1,FC=1.91),and isocitrate dehydrogenase 1(IDH1,FC=8.12).The metabolic processes associated with ammonia detoxification and antioxidant stress were also affected by starter diet supplementation,with proteins,microsomal glutathione S-transferase 3(MGST3,FC=2.37)and IDH1,linked to the biosynthesis of glutamate and glutathione metabolism pathway being upregulated in the group with starter diet *** addition,starter feeding decreased the expression of Rasrelated protein rap-1 A(RAP1 A,FC=0.48)enriched in Rap1 signaling pathway,Ras signaling pathway,cyclic adenosine monophosphate(cAMP)signaling pa