Serum trimethylamine-N-oxide and gut microbiome alterations are associated with cholesterol deposition in the liver of laying hens fed with rapeseed meal

作     者：Liping Zhu Jianping Wang Xuemei Ding Shiping Bai Qiufeng Zeng Yue Xuan Gregory S.Fraley Keying Zhang Liping Zhu;Jianping Wang;Xuemei Ding;Shiping Bai;Qiufeng Zeng;Yue Xuan;Gregory S.Fraley;Keying Zhang

作者机构：Animal Nutrition InstituteKey Laboratory for Animal Disease-Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural UniversityChengduSichuan611130China Department of BiologyHope CollegeHollandMI49423USA 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第4期

页      面：1258-1270页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：fanatically supported by National Science and Technology Project(2014BAD13B04) the Sichuan Provincial Science and Technology project(2018NZ20009,2014NZ0043) 

主　　题：Sinapine Expeller rapeseed meal Trimethylamine N-oxide Liver lipid Cecum microbiota Laying hen 

摘      要：Sinapine derived from cruciferous plants could be converted into trimethylamine by intestinal *** metabolite,trimethylamine N-oxide(TMAO),is closely linked to increased risk of cardiovascular disease and fat deposition in *** fed with rapeseed meal(RSM)suffered from fatty liver hemorrhage syndrome(FLHS).This study was conducted to investigate whether RSM-induced fatty liver is due to TMAO via altering microbiota composition and *** 33 weeks of age,600 laying hens were randomly divided into 5 treatment groups,namely control and 14%RSM treatment groups(DY5,with 16.2% erucic acid [EA]and 74.66% glucosinolate[Gl]contents;MB1,with 3.50% EA and 43.23 %Gl contents;DY6,with 6.7% EA and 22.67% Gl contents;XH3,with 44.60% EA and 132.83% Gl contents)for 8 *** revealed that 3 hens died due to liver hemorrhage after ingesting 14% RSM *** 14% RSM decreased serum low-density lipoprotein cholesterol(LDL-C)content(P0.01)while tended to increase serum TMAO content compared to the control group(P=0.08).The 14% RSM diet increased red oil O optical density(P0.01),and increased total cholesterol(TC)and LDL-C content in the liver(P0.01,and P0.01,respectively).The 14%RSM decreased liver total bile acid(TBA)content compared to the control(P0.01).The DY6 had a higher TBA content in the liver than the XH3(P0.01).The 14%RSM decreased mRNA abundance of liver X receptors alpha(LXR-α,P=0.01),and increased mRNA abundance of sterol response element binding protein 2(SREBP-2,P=0.04).Results revealed that the in-feed RSM could alter richness and diversity of cecal microbiota compared to the control(P0.05).Liver TC content and serum TMAO showed a negative relationship with Proteobacteria and Actinobacteria(P=0.04).In conclusion,14%RSM increased liver TC and induced high liver score of FLHS,which was possibly associated with the altered cecal microbiota composition,increased serum TMAO levels and LXR-αand SREBP-2 expressions.