Dynamic changes of genomic methylation profiles at different growth stages in Chinese Tan sheep

作     者：Yufang Liu Qiao Xu Xiaolong Kang Kejun Wang Jve Wang Dengzhen Feng Ying Bai Meiying Fang Yufang Liu;Qiao Xu;Xiaolong Kang;Kejun Wang;Jve Wang;Dengzhen Feng;Ying Bai;Meiying Fang

作者机构：Department of Animal Genetics and BreedingNational Engineering Laboratory for Animal BreedingMOA Laboratory of Animal Genetics and BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityNo.2 Yuanmingyuan West RdBeijing 100193People’s Republic of China College of Life Sciences and Food EngineeringHebei University of EngineeringHandan 056021People’s Republic of China Biotechnology InstituteNanchang Normal UniversityNanchang 330029People’s Republic of China College of AgricultureNingxia UniversityYinchuan 750021People’s Republic of China College of Animal Science and Veterinary MedicineHenan Agricultural UniversityZhengzhou 450002People’s Republic of China Beijing Key Laboratory for Animal Genetic ImprovementBeijing 100193People’s Republic of China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第1期

页      面：82-93页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported by the talent cultivation and developmental support program of China Agricultural University,an award to study the cultivation of high-quality mutton sheep varieties(or lines)from Ningxia province(NXNYYZ20150101) the Natural Science Foundation of Hebei Province of China for Youths(C2019402261) 

主　　题：Chinese tan sheep Different growth stages DNA methylation Whole genome bisulfite sequencing 

摘      要：Background:Tan sheep,an important local sheep breed in China,is famous for their fur ***-month-old Tan sheep have white,curly hair with beautiful flower spikes,commonly known as“nine bends,which has high economic ***,the“nine bendscharacteristic gradually disappears with age;consequently,the economic value of the Tan sheep ***-related changes in DNA methylation have been reported and may be responsible for age-induced changes in gene *** now,no genome-wide surveys have been conducted to identify potential DNA methylation sites involved in different sheep growth *** this study we investigated the dynamic changes of genome-wide DNA methylation profiles in Tan sheep using DNA from skin and deep whole-genome bisulfite sequencing,and compared the DNA methylation levels at three different growth stages:1,24,and 48 months old(mon1,mon24,and mon48,respectively).Results:In this study,11 skin samples from three growth stages(four for mon1,four for mon24,and three for mon48)were used for DNA methylation analysis and gene expression *** were 52,288 and 236 differentially methylated genes(DMGs)identified between mon1 and mon24,mon1 and mon48,and mon24 and mon48,*** the differentially methylated regions,1.11%,7.61%,and 7.65% were in the promoter in mon1 ***24,mon24 ***48,and mon1 ***48,*** were enriched in the MAPK and WNT signaling pathways,which are related to age growth and hair follicle morphogenesis *** were 51 DMGs associated with age growth and curly fleece *** DMGs between mon1 and mon48(KRT71,CD44,ROR2 and ZDHHC13)were further validated by bisulfite ***:This study revealed dynamic changes in the genomic methylation profiles of mon1,mon24,and mon48 sheep,and the percentages of methylated cytosines were 3.38%,2.85% and 4.17%,*** the DMGs,KRT71 and CD44 were highly methylated in mon1,and ROR2 and ZDHHC13 were highly methylated