Mass Spectrometry Analysis of SARS-CoV-2 Nucleocapsid Protein Reveals Camouflaging Glycans and Unique Post-Translational Modifications
作者机构：State Key Laboratory for Diagnosis and Treatment of Infectious DiseasesNational Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseThe First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiangChina
出 版 物：《Infectious Microbes & Diseases》 (感染微生物与疾病（英文）)
年 卷 期：2021年第3卷第3期
页 面：149-157页
学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 081704[工学-应用化学] 1002[医学-临床医学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0703[理学-化学] 10[医学]
基 金：supported by the National Natural Science Foundation of China(U20A20343) the National Key Research and Development Program(2017YFC1200204) Emergency Project of Zhejiang Provincial Department of Science and Technology(2020C03123-1) Fundamental Research Funds for the Central Universities(2018XZZX001-13)
主 题：glycosylation mass spectrometry nucleocapsid protein post-translational modification SARS-CoV-2
摘 要：The devastating coronavirus disease 2019(COVID-19)pandemic has prompted worldwide efforts to study structural biological traits of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its viral *** to the Spike protein,which is the primary target for currently available vaccines or antibodies,knowledge about other virion structural components is *** high-resolution mass spectrometry,we report a comprehensive post-translational modification(PTM)analysis of nucleocapsid phosphoprotein(NCP),the most abundant structural component of the SARS-CoV-2 *** addition to phosphoryl groups,we show that the SARS-CoV-2 NCP is decorated with a variety of PTMs,including N-glycans and *** on newly identified PTMs,refined protein structural models of SARS-CoV-2 NCP were proposed and potential immune recognition epitopes of NCP were aligned with *** data can facilitate the design of novel vaccines or therapeutics targeting NCP,as valuable alternatives to the current vaccination and treatment paradigm that is under threat of the ever-mutating SARS-CoV-2 Spike protein.
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