Effects of Three-Dimensional Platform Stiffness and Layer Dimensions on Separation of Carcinoma Cells

作     者：W.G.Zhang Z.Y.Liu S.W.Pang W.G.Zhang;Z.Y.Liu;S.W.Pang

作者机构：Department of Electrical EngineeringCenter for BiosystemsNeuroscienceand NanotechnologyCity University of Hong KongHong Kong 999077China 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2021年第7卷第10期

页      面：1424-1433页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by the Center for Biosystems,Neuroscience,and Nanotechnology(CBNN)of City University of Hong Kong(9360148 and 9380062) the University Grants Council of Hong Kong(GRF projects:11247716,11218017,11213018,and 11212519,CRF project:C1013-15G) 

主　　题：Cell separation Cell migration Nasopharyngeal carcinoma(NPC) 3D scaffold platform 

摘      要：Cancer cell separation is highly desirable for cancer diagnosis and *** biochemical methods,engineered platforms are effective alternatives for sorting carcinoma cells from normal cells based on their unique properties in responding to the physical changes of the surrounding *** this work,three-dimensional(3D)biomimetic scaffold platforms were developed to separate nasopharyngeal carcinoma 43(NPC43)cells from immortalized nasopharyngeal epithelial 460(NP460)cells based on precisely controlled design parameters including stiffness,number of layers,and structural *** migration characteristics of NPC43 and NP460 cells on the scaffold platforms revealed that NPC43 cells could squeeze into 10 lm wide,15 lm deep trenches while NP460 cells could *** different migration behavior was mainly due to cells having different interactions with the surrounding ***43 cells had filopodia-like protrusions,while NP460 cells exhibited a sheet-like *** these 3D biomimetic platforms,89%separation efficiency of NPC43 cells from NP460 cells was achieved on stiffer two-layer scaffold platforms with a 40/10 lm ridge/trench(R/T)grating on the top layer and a 20/10 lm R/T grid on the bottom ***,the separation efficiency was further increased to 93%by adding an active conditioned medium(ACM)that caused the cells to have higher motility and *** results demonstrate the capability to apply biomimetic engineered platforms with appropriate designs to separate cancer cells from normal cells for potential cancer diagnosis and treatment.