Bystander Effects on Mammalian Cells Induced by Carbon-ion-radiation

作     者：Wang Jufang, Li Wenjian, Dang Bingrong and Ma Qiufeng 

出 版 物：《近代物理研究所和兰州重离子加速器实验室年报：英文版》 (IMP & HIRFL Annual Report)

年 卷 期：2004年第1期

页      面：90-90页

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：哺乳动物细胞 碳离子辐照 离子辐射 条件培养基 ICM方法 诱导 旁观者效应 V79细胞 

摘      要：Bystander effects on non-irradiated V79 cells were observed by irradiated conditioned medium (ICM) method and co-cultured with carbon-ion-irradiated V79 cells. In the ICM method, cells were irradiated with 1 Gy of carbon ions, cultured with new fresh medium at 37℃for 1 and 2 h. The medium was collected and filtered with 0.22 μm film to remove cells irradiated completely. This medium was transferred into culture dishes with normal cells. After cultured for 7 d, cell colonies were counted. The results were shown in Tab.1, the