HUMAN CHROMOSOME BANDING WITH RESTRICTION ENDONUCLEASES HaeIII,HinfI AND PstI

作     者：张思仲 董伟峰 ZHANG SI-ZHONG;DONG WEI-FENG Department of Medical Genetics, West China University of Medical Sciences, Chengdu 610044, PRC

作者机构：Department of Medical Genetics West China University of Medical Sciences Chengdu 610044 PRC Department of Medical Genetics West China University of Medical Sciences Chengdu 610044 PRC Human chromosome banding was carried out with restriction endonucleases (REs)HaeⅢ HinfⅠ PstⅠand the effects of some factors on it were examined. HaeⅢinduced Cand G banding patterns HinfⅠinduced negative C bands but the centromeric heterochro-matin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphismof C banded region was discovered in chromosome 4. PstⅠinduced G-like banding pattern.The duration of enzymatic digestion the concentration of glycerol in the reaction bufferas well as the ageing and heating of chromosome preparations all had a significant influ-ence on the banding effects of the REs. 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1989年第32卷第11期

页      面：1350-1360页

核心收录：

学科分类：0810[工学-信息与通信工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0708[理学-地球物理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0704[理学-天文学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：Project supported by National Natural Science Foundation of China 

主　　题：restriction endonucleases human chromosome banding 

摘      要：Human chromosome banding was carried out with restriction endonucleases (REs)HaeⅢ, HinfⅠ, PstⅠ and the effects of some factors on it were examined. HaeⅢ induced Cand G banding patterns, HinfⅠ induced negative C bands but the centromeric heterochro-matin of chromosomes 3 and 4 remained selectively dark-stained. A new heteromorphismof C banded region was discovered in chromosome 4. PstⅠ induced G-like banding *** duration of enzymatic digestion, the concentration of glycerol in the reaction buffer,as well as the ageing and heating of chromosome preparations all had a significant influ-ence on the banding effects of the REs.