A Smart Procedure for the Femtosecond Laser-Based Fabrication of a Polymeric Lab-on-a-Chip for Capturing Tumor Cell

作     者：Annalisa Volpe Udith Krishnan Maria Serena Chiriacò Elisabetta Primiceri Antonio Ancona Francesco Ferrara Annalisa Volpe;Udith Krishnan;Maria Serena Chiriacò;Elisabetta Primiceri;Antonio Ancona;Francesco Ferrara

作者机构：Department of PhysicsUniversity of Bari“Aldo Moro”Bari 70126Italy Institute for Photonics and NanotechnologiesBari 70126Italy Institute of NanotechnologyLecce 73100Italy STMicroelectronicsLecce 73100Italy 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2021年第7卷第10期

页      面：1434-1440页

核心收录：

学科分类：080903[工学-微电子学与固体电子学] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by SMILE(a SAW-MIP Integrated device for oraL cancer Early detection)project part of the ATTRACT program that has received funding from the European Union’s Horizon 2020 Research and Innovation Program(777222) 

主　　题：Lab-on-a-chip Fs laser Circulating tumor cells Point of care Thermal bonding Polymers 

摘      要：Rapid prototyping methods for the design and fabrication of polymeric labs-on-a-chip are on the rise,as they allow high degrees of precision and *** example,a microfluidic platform may require an optimization phase in which it could be necessary to continuously modify the architecture and geometry;however,this is only possible if easy,controllable fabrication methods and low-cost materials are *** this paper,we describe the realization process of a microfluidic tool,from the computer-aided design(CAD)to the proof-of-concept application as a capture device for circulating tumor cells(CTCs).The entire platform was realized in polymethyl methacrylate(PMMA),combining femtosecond(fs)laser and micromilling fabrication *** multilayer device was assembled through a facile and low-cost solvent-assisted method.A serpentine microchannel was then directly biofunctionalized by immobilizing capture probes able to distinguish cancer from non-cancer cells without *** low material costs,customizable methods,and biological application of the realized platform make it a suitable model for industrial exploitation and applications at the point of care.