Transcriptomic characteristics and impaired immune function of patients who retest positive for SARS-CoV-2 RNA

作     者：Dongyao Wang Dong Wang Min Huang Xiaohu Zheng Yiqing Shen Binqing Fu Hong Zhao Xianxiang Chen Peng Peng Qi Zhu Yonggang Zhou Jinghe Zhang Zhigang Tian Wuxiang Guan Guiqiang Wang Haiming Wei Dongyao Wang;Dong Wang;Min Huang;Xiaohu Zheng;Yiqing Shen;Binqing Fu;Hong Zhao;Xianxiang Chen;Peng Peng;Qi Zhu;Yonggang Zhou;Jinghe Zhang;Zhigang Tian;Wuxiang Guan;Guiqiang Wang;Haiming Wei

作者机构：Division of Life Sciences and MedicineDepartment of HematologyThe First Affiliated Hospital of University of Science and Technology of ChinaUniversity of Science and Technology of ChinaHefei 230001China Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medicine and Medical CenterUniversity of Science and Technology of ChinaHefei 230001China Hefei National Laboratory for Physical Sciences at MicroscaleUniversity of Science and Technology of ChinaHefei 230001China Department of Laboratory MedicineTongji HospitalTongji Medical CollegeHuaZhong University of Science and TechnologyWuhan 430030China Department of Infectious DiseasesPeking University First HospitalBeijing 100034China Department of TuberculosisWuhan Pulmonary HospitalWuhan 430030China Center for Emerging Infectious DiseasesWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Peking University International HospitalBeijing 100034China 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2021年第13卷第10期

页      面：748-759页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by grants from the China National Center for Biotechnology Development(2020YFC0843800 and 2020YFC0846800) the Ministry of Science and Technology of China(2020TFC0844100) China Postdoctoral Science Foundation(2020T130112ZX) Postdoctoral Foundation of Hefei(2020130) 

主　　题：COVID-19 CD8f T cells NK cells RTP patients immune function 

摘      要：Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,has become a global public health *** patients who have recovered from COVID-19 subsequently test positive again for SARS-CoV-2 RNA after discharge from *** such retest-positive(RTP)patients become infected again is not *** this study,30 RTP patients,20 convalescent patients,and 20 healthy controls were enrolled for the analysis of immunological characteristics of their peripheral blood mononuclear *** found that absolute numbers of CD4^(+)T cells,CD8^(+)T cells,and natural killer cells were not substantially decreased in RTP patients,but the expression of activation markers on these cells was significantly *** percentage of granzyme B-producing T cells was also lower in RTP patients than in convalescent *** transcriptome sequencing,we demonstrated that high expression of inhibitor of differentiation 1(ID1)and low expression of interferon-induced transmembrane protein 10(IFITM10)were associated with insufficient activation of immune cells and the occurrence of *** findings provide insight into the impaired immune function associated with COVID-19 and the pathogenesis of RTP,which may contribute to a better understanding of the mechanisms underlying RTP.