Characterization and mode of action analysis of black soldier fly(Hermetia illucens)larva-derived hemocytes
作者机构：Justus-Liebig-Universität GießenInstitut für Allgemeine Zoologie und EntwicklungsbiologieZelluläre Erkennungs-und AbwehrprozesseGießenGermany Technische Universität DresdenFakultät BiologieInstitut für ZoologieDresdenGermany Technische Universität DresdenFakultät BiologieInstitut für MikrobiologieDresdenGermany Present address:Philipp F.PoppInstitute for Biology-Bacterial PhysiologyHumboldt-Universität zu BerlinBerlinGermany
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2022年第29卷第4期
页 面：1071-1095页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：We wish to thank Prof.Herwig Gutzeit for valuable discussions and providing antibody stocks.We would like to express our gratitude to Dr.Alexander Froschauer and Prof.Klaus Reinhardt who hosted the work in their laboratories to Prof.Thorsten Mascher and Prof.Christian Dahmann for allowing us to use their laboratory equipment and Dr.Diana Wolf who provided bacteria strains.We thank Dr.Uwe Töpfer for valuable discussions and assistance in microscopy Dennis Höfling for Hermetia breeding and Katharina Starke Yvonne Henker and Christin Froschauer for assistance in the laboratory.We thank the two anonymous reviewers for their helpful comments that improved the quality of the manuscript significantly
主 题：black soldier fly(Hermetia ilucens) cellular immunity crystal cell hemocyte types immunity of economically imporant insects microbe phagoeytosis plasmatocyte
摘 要：With the growing importance of the black soldier fly(Hermetia illucens)for both sustainable food production and waste management as well as for science,a great demand of understanding its immune system ***,we present the first description of the circulating larval hemocytes with special emphasis on uptake of microorganisms and distinguishing hemocyte *** histological,zymographic,and cytometric methods and with a set of hemocyte binding lectins and antibodies,the hemocytes of *** are identified as plasmatocytes,crystal cells,and putative *** hemocyte counts(THC)are determined,and methods for THC determination are *** 1100 hemocytes per microliter hemolymph are present in naive animals,while hemocyte density decreases dramatically shortly after wounding,indicating a role of hemocytes in response to wounding(and immune response in general).The determination of the relative abundance of each hemocyte type(differential hemocyte count,DHC)revealed that plasmatocytes are highly abundant,whereas prohemocytes and crystal cells make up only a small percentage of the circulating *** are not only the most abundant but also the professional phagocytes in *** rapidly engulf and take up bacteria both in vivo and in vitro,indicating a very potent cellular defense against invading *** bioparticles such as yeasts are also removed from circulation by phagocytosis,but slower than *** is the first analysis of the potent cellular immune response in the black soldier fly,and a first toolbox that helps to identify hemocyte(types)is presented.
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