Effect of Roucongrong(Herba Cistanches Deserticolae) decoction on the substantia nigra through Wnt/β-catenin signaling pathway in rats with Parkinson's disease induced by 6-hydroxydopamine hydrochloride

作     者：XU Qian QIN Wei WU Fangzhen LIN Yao HONG Liting CHEN Dan HU Xuefeng CAI Jing XU Qian;QIN Wei;WU Fangzhen;LIN Yao;HONG Liting;CHEN Dan;HU Xuefeng;CAI Jing

作者机构：Fujian Key Laboratory of Developmental and Neural BiologyCollege of Life ScienceFujian Normal UniversityFuzhou 350117China College of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou 350122China Department of Rheumatology and EndocrinologyThe Second Affiliated Hospital of Fujian Traditional Chinese Medical UniversityFuzhou 350003China Institute of PharmacyFujian University of Traditional Chinese MedicineFuzhou 350122China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2021年第41卷第5期

页      面：762-770页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Creation of Major New Drugs for Major National Science and Technology Projects(No.2019ZX09301154) National Natural Science Foundation of China(Nos.81904263,81804156) 2021 Fujian Provincial Traditional Chinese Medicine Science and Technology Program 2018 Outstanding Young Scientific Research Project in Fujian province 

主　　题：Cistanche Parkinson disease survival tyrosine 3-monooxygenase Wnt signaling pathway 

摘      要：OBJECTIVE:To investigate the effect of Roucongrong(Herba Cistanches Deserticolae)decoction on the substantia nigra in rats with Parkinson’s disease(PD)induced by 6-hydroxydopamine hydrochloride(6-OHDA).To further determine whether the Wnt/β-catenin signaling pathway is involved in the ***:A rat model of PD was established by intracranial injection of ***,three concentrations of Roucongrong(Herba Cistanches Deserticolae)decoction were prepared and administered to rats by gavage therapy for 14 *** changes were measured in PD *** vivo tyrosine hydroxylase(TH)levels in the substantia nigra were examined by ***,gene and protein expression levels of members of the Wnt/β-catenin signaling pathway were examined by Western blotting and polymerase chain ***,a Wnt/β-catenin inhibitor was used to investigate the mechanism of action in 1-methyl-4-phenylpyridinium(MPP+)-treated MES23.5 cells in ***:Roucongrong(Herba Cistanches Deserticolae)decoction improved performance in the stride and gait adjustment tests in PD *** also increased TH in the substantia nigra and altered the expression of genes and proteins in the Wnt/β-catenin signaling ***/β-catenin inhibitor reduced the effect of Roucongrong(Herba Cistanches Deserticolae)decoction in MPP+-treated MES23.5 ***:Roucongrong(Herba Cistanches Deserticolae)decoction may promote neuronal survival in PD in vivo and in vitro by increasing TH content in the substantia nigra and by activating the Wnt/β-catenin signaling pathway.