MouseVenue3D:A Markerless Three-Dimension Behavioral Tracking System for Matching Two-Photon Brain Imaging in Free-Moving Mice

作     者：Yaning Han Kang Huang Ke Chen Hongli Pan Furong Ju Yueyue Long Gao Gao Runlong Wu Aimin Wang Liping Wang Pengfei Wei 

作者机构：Shenzhen Key Laboratory of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain ScienceGuangdong Provincial Key Laboratory of Brain Connectome and BehaviorCAS Center for Excellence in Brain Science and Intelligence TechnologyBrain Cognition and Brain Disease InstituteShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen-Hong Kong Institute of Brain ScienceShenzhen Fundamental Research InstitutionsShenzhen518055China University of Chinese Academy of SciencesBeijing100049China University of RochesterRochesterNY14627USA Honam UniversityGwangju62399South Korea State Key Laboratory of Membrane BiologyInstitute of Molecular MedicinePeking UniversityBeijing100101China Department of ElectronicsPeking UniversityBeijing100871China State Key Laboratory of Advanced Optical Communication Systems and NetworksPeking UniversityBeijing100101China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2022年第38卷第3期

页      面：303-317页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071006[理学-神经生物学] 

基　　金：the Key Area R&D Program of Guangdong Province,China(2018B030338001 and 2018B030331001) the National Key R&D Program of China(2018YFA0701403) the National Natural Science Foundation of China(31500861,31630031,91732304,and 31930047) Chang Jiang Scholars Program,the International Big Science Program Cultivating Project of the Chinese Academy of Science(CAS 172644KYS820170004) the Strategic Priority Research Program of the CAS(XDB32030100) the Youth Innovation Promotion Association of the CAS(2017413) the CAS Key Laboratory of Brain Connectome and Manipulation(2019DP173024) Shenzhen Government Basic Research Grants(JCYJ20170411140807570,JCYJ20170413164535041) the Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20160429185235132) a Helmholtz-CAS Joint Research grant(GJHZ1508) Guangdong Provincial Key Laboratory of Brain Connectome and Behavior(2017B030301017) the Ten Thousand Talent Program,the Guangdong Special Support Program,Key Laboratory of Shenzhen Institute of Advanced Technology(2019DP173024) the Shenzhen Key Science and Technology Infrastructure Planning Project(ZDKJ20190204002) 

主　　题：Computational neuroethology Behavioral and neural recording 3-D pose estimation Multi-view cameras Automatic calibration 

摘      要：Understanding the connection between brain and behavior in animals requires precise monitoring of their behaviors in three-dimensional(3-D)***,there is no available three-dimensional behavior capture system that focuses on ***,we present MouseVenue3D,an automated and low-cost system for the efficient capture of 3-D skeleton trajectories in markerless *** improved the most time-consuming step in 3-D behavior capturing by developing an automatic calibration ***,we validated this process in behavior recognition tasks,and showed that 3-D behavioral data achieved higher accuracy than 2-D ***,MouseVenue3D was combined with fast high-resolution miniature two-photon microscopy for synchronous neural recording and behavioral tracking in the freely-moving ***,we successfully decoded spontaneous neuronal activity from the 3-D behavior of *** findings reveal that subtle,spontaneous behavior modules are strongly correlated with spontaneous neuronal activity patterns.