Expression of toll-like receptor 4 gene polymorphism and inflammatory factors in peripheral blood on patients with sepsis

作     者：Tieying Tian Xiyuan Xu Jingping Yang 

作者机构：Department of Respiratory and Critical Medicinethe Third Affiliated Hospital of Inner Mongolia Medical University(Baogang Hospital)BaotouInner MongoliaChina 

出 版 物：《Discussion of Clinical Cases》 (临床病例讨论杂志（英文版）)

年 卷 期：2020年第7卷第4期

页      面：15-21页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Sepsis Toll-like receptor 4 Interleukin-23 Gene polymorphism 

摘      要：Objective:To observe the presence of toll-like receptor 4(TLR4)gene polymorphism in the venous blood in patients with sepsis,meanwhile,to observe the expression and the diagnostic value of TLR4 mRNA,interferon-(IFN-),interleukin-23(IL-23),procalcitonin(PCT)and C-reactive protein(CRP)in patients with different degrees of ***:The peripheral blood samples from the subjects were collected to extract genomic DNA,gene sequencing and enzyme digestion method were applied to the detection of TLR4 Asp299Gly loci *** expression of TLR4 mRNA in the peripheral blood was detected by reverse transcriptase polymerase chain reaction(RT-PCR).The expression levels of IFN-,IL-23,PCT and CRP were measured by enzyme linked immunosorbent assay(ELISA).Results:(1)The gene polymorphism was not present in TLR4 Asp299Gly loci;(2)The expression of TLR4 mRNA in the peripheral blood in patients with sepsis:on d1,there were statistically significant differences between the normal group and the group(APACHE II≤20),between the normal group and the group(APACHE II20),between the group(APACHE II≤20)and the group(AAPACHE II20)(t=5.741,14.780 and 10.500,all p20),between the group(APACHE II≤20)and the group(APACHE II20)(t=4.186,13.830 and 9.645,all p.01);(3)The expression levels of IFN-,IL-23,PCT and CRP were obviously upregulated(all p.01),and TLR4 was positively correlated with IFN-and IL-23(all p.01);(4)The best cutoff value of TLR4 mRNA at baseline was 891.6μg/L,with a sensitivity of 100%and a diagnostic specificity of 57%.The best cutoff value of IFN-at baseline was 84.5μg/L,with a sensitivity of 100%and a diagnostic specificity of 57%.The best cutoff value of IL-23 at baseline was 861μg/L,with a sensitivity of 100%and a diagnostic specific