Ang-(1-7)exerts anti-inflammatory and antioxidant activities on high glucose-induced injury by prohibiting NF-κB-IL-1βand activating HO-1 pathways in HUVECs

作     者：FEI CHENG YIQIAN DING QING XU WEI ZHANG YULAN ZHEN JING LIU SHICHENG LI CHANG TU GUOHUA LAI JUN LAN JINGFU CHEN 

作者机构：Department of Cardiovascular MedicineDongguan Songshan Lake Center HospitalDongguan Cardiovascular InstituteDongguan Shilong People’s Hospital Affiliated to Southern Medical UniversityDongguan523326China Department of UltrasoundThe First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of CardiologyHuangpu Division of the First Affiliated HospitalSun Yat-sen UniversityGuangzhou510700China Department of OncologyDongguan Third People’s HospitalDongguan523326China Second Ward of Internal MedicineDongguan Eighth People’s HospitalDongguan Children’s HospitalDongguan523003China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第4期

页      面：1053-1066页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Technology Planning Project of Huangpu District(201544-01) Medical Scientific Research Foundation of Guangdong Province(A2015287) Science and Technology Planning Project of Guangdong Province(2017ZC0474) Natural Science Foundation of Guangdong Province(2015A030313690) General Project of Dongguan City(Nos.201950715024922 and 2018507150241344) 

主　　题：Angiotensin-(1-7) High glucose Human umbilical vein endothelial cells NF-κB IL-1β HO-1 

摘      要：Previous reports have suggested that Ang-(1-7)may have a protective effect in endothelial cells against high glucose(HG)-induced cell injury thanks to a modulatory mechanism in the NF-κB signaling *** this study,we have examined whether NF-κB-IL-1βand Heme oxygenase-1(HO-1)pathways contribute to the protection of Ang-(1-7)against hyperglycemia-induced inflammation and oxidative stress in human umbilical vein endothelial cells(HUVECs).Our results indicate that time-varying exposures of HUVECs,from 1 h to 24 h,to high glucose concentrations result in an increased expression of phosphorylated(p)-p65 and HO-1 in a time-dependent *** an inhibitor of NF-κB,pyrrolidinedithiocarbamic acid(PDTC)suppressed IL-1βproduction induced by *** note,HUVECs previously treated with Ang-(1-7)(2μM)for 30 min before being exposed to HG concentrations significantly ameliorated the HG-increased in p-p65 and IL-1βexpression;whereas obviously up-regulated the level of HO-1,along with inhibition of oxidative stress,inflammation,and the HG-induced ***,when HUVECs were previously treated either with PDTC or IL-1Ra for 30 min before being exposed to HG,it significantly prevented damages caused by high glucose concentrations mentioned above,while the treatment of HO-1 inhibitor Sn-protoporphyrin(SnPP)before exposure to both HG and Ang-(1-7)significantly blocked the protective effect exerted by Ang-(1-7)on endothelial cells against injuries induced by HG mentioned *** conclude,the data of this study showed that activation and inhibition of the NF-κB-IL-1βpathway and HO-1 pathway may constitute an important defense mechanism against endothelial cell damage caused by HG *** additionally gave new evidence showing that exogenous Ang-(1-7)exerts a protective effect on HUVECs against the HG-induced cell injury via the inhibition and the activation of the NF-κB-IL-1βpathway and the HO-1 pathway,respectively.