Evolution-aided engineering of plant specialized metabolism
作者机构：Plant Biology SectionSchool of Integrative Plant ScienceCornell UniversityIthacaNYUSA Metabolic Diversity GroupLeibniz Institute of Plant Genetics and Crop Plant Research(IPK)06466 SeelandGermany
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2021年第2卷第3期
页 面：240-263页
核心收录：
学科分类：09[农学] 0903[农学-农业资源与环境] 090302[农学-植物营养学]
基 金：funding to BC, PR, JD from the state of Sachsen-Anhalt and EFRE(Europaischer Fonds fur regionale Entwicklung) the United States Department of Agriculture Hatch project HyperSpEED(Hypericum multispecies Exploration of Extracts Diversity) The project is part of the research cluster Autonomie im Alter(AiA)
主 题：Evolution Metabolic engineering Plant biotechnology Synthetic biology
摘 要：The evolution of new traits in living organisms occurs via the processes of mutation,recombination,genetic drift,and *** processes that have resulted in the immense biol ogical diversity on our planet are also being employed in metabolic engineering to optimize enzymes and pathways,create new-to-nature reactions,and synthesize complex natural products in heterologous *** this review,we discuss two evolution-aided strategies for metabolic engineering-directed evolution,which improves upon existing genetic templates using the evolutionary process,and combinatorial pathway reconstruction,which brings together genes evolved in different organisms into a single heterol ogous *** discuss the general principles of these strategies,describe the technologies involved and the molecular traits they influence,provide examples of their use,and discuss the roadblocks that need to be addressed for their wider adoption.A better understanding of these strategies can provide an impetus to research on gene function discovery and biochemical evolution,which is foundational for improved metabolic *** evolution-aided approaches thus have a substantial potential for improving our understanding of plant metabolism in general,for enhancing the production of plant metabolites,and in sustainable agriculture.
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