Korean red ginseng decreases 1-methyl-4-phenylpyridinium-induced mitophagy in SH-SY5Y cells

作     者：Hyongjun Jeon Hee-Young Kim Chang-Hwan Bae Yukyung Lee Sungtae Koo Seungtae Kim Hyongjun Jeon;Hee-Young Kim;Chang-Hwan Bae;Yukyung Lee;Sungtae Koo;Seungtae Kim

作者机构：Department of Korean Medical ScienceSchool of Korean MedicinePusan National UniversityYangsanGyeongsangnam-do 50612Republic of Korea Korean Medicine Research Center for Healthy AgingPusan National UniversityYangsanGyeongsangnam-do 50612Republic of Korea 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2021年第19卷第6期

页      面：537-544页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：financially supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Korea government(MSIT)(No.NRF-2019R1A2C1085130) 

主　　题：Parkinson’s disease Apoptosis Mitophagy 1-Methyl-4-phenylpyridinium Korean red ginseng 

摘      要：Objective:Mitophagy is known to contribute towards progression of Parkinson’s *** red ginseng(KRG)is a widely used medicinal herb in East Asia,and recent studies have reported that KRG prevents 1-methyl-4-phenylpyridinium ion(MPP^(+))-induced cell *** study was undertaken to investigate whether KRG suppresses MPP^(+)-induced apoptosis and ***:SH-SY5 Y cells were incubated with KRG for 24 h,and subsequently exposed to MPP^(+).The MPP^(+)-induced cell death was confirmed with the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay,and the terminal deoxynucleotidyl transferase-mediated d UTP nick end-labeling *** in the structure and function of mitochondria were confirmed using mitotracker,Mito SOX red mitochondrial superoxide indicator,parkin,and phosphatase and tensin homolog deleted on chromosome ten-induced putative kinase 1(PINK1)immunofluorescent *** blotting was performed to evaluate the expression of apoptosis-related factors in whole cells,including Bax,Bcl-2 and cleaved caspase-3,and mitophagy-related factors in the mitochondrial fraction,including cytochrome c,parkin,PINK1,translocase of the outer membrane 20(TOM20),p62 and Beclin ***:MPP^(+)induced cell death by cytochrome c release and caspase-3 activation;however,this effect was suppressed by KRG’s regulation of the expressions of Bcl-2 and ***,MPP^(+)exposure increased the mitochondrial expressions of parkin,PINK1,Beclin 1 and p62,and decreased TOM20,cytochrome c and Bcl-2 *** MPP^(+)-induced changes in the mitochondrial fraction were attenuated by treatment with ***:KRG effectively prevents MPP^(+)-induced SH-SY5 Y cell death by regulating cytochrome c release from mitochondria and PINK1/parkin-mediated mitophagy,through regulation of the Bcl-2 family.