HOXA11-AS aggravates microglia-induced neuroinflammation after traumatic brain injury

作     者：Xiang-Long Li Bin Wang Fu-Bing Yang Li-Gang Chen Jian You Xiang-Long Li;Bin Wang;Fu-Bing Yang;Li-Gang Chen;Jian You

作者机构：Department of Neurosurgerythe Affiliated Hospital of Southwest Medical UniversityLuzhouSichuan ProvinceChina Neurosurgical Clinical Research Center and Academician(Expert)Workstation of Sichuan ProvinceLuzhouSichuan ProvinceChina Laboratory of Neurological Diseases and Brain Functionsthe Affiliated Hospital of Southwest Medical UniversityLuzhouSichuan ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2022年第17卷第5期

页      面：1096-1105页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the Science and Technology Project of Sichuan Province of China,No.2020YJ0188 the Science and Technology Foundation of Luzhou of China,No.2017LZXNYD-J10(both to XLL) 

主　　题：astrocyte competitive endogenous RNA HOXA11-AS microglia midkine miR-124-3p neuroinflammation traumatic brain injury 

摘      要：Long noncoding RNAs(lnc RNAs)participate in many pathophysiological processes after traumatic brain injury by mediating neuroinflammation and *** A11 antisense RNA(HOXA11-AS)is a member of the lnc RNA family that has been reported to participate in many inflammatory reactions;however,its role in traumatic brain injury remains *** this study,we established rat models of traumatic brain injury using a weight-drop hitting device and injected LV-HOXA11-AS into the right lateral ventricle 2 weeks before *** results revealed that overexpression of HOXA11-AS aggravated neurological deficits in traumatic brain injury rats,increased brain edema and apoptosis,promoted the secretion of proinflammatory factors interleukin-1β,interleukin-6,and tumor necrosis factorα,and promoted the activation of astrocytes and *** were treated with 100 ng/m L lipopolysaccharide for 24 hours to establish in vitro cell models,and then transfected with pc DNA-HOXA11-AS,mi R-124-3 p mimic,or *** results revealed that HOXA11-AS inhibited mi R-124-3 p expression and boosted MDK expression and TLR4-nuclear factor-κB pathway ***,lipopolysaccharide enhanced potent microglia-induced inflammatory responses in *** overexpression of mi R-124-3 p or downregulating MDK repressed microglial activation and the inflammatory response of ***,the mi R-124-3 p-mediated anti-inflammatory effects were reversed by *** findings suggest that HOXA11-AS can aggravate neuroinflammation after traumatic brain injury by modulating the mi R-124-3 p-MDK *** study was approved by the Animal Protection and Use Committee of Southwest Medical University(approval ***-2019-042)on February 4,2019.