Verification of Lactobacillus brevis tolerance to simulated gastric juice and the potential effects of postbiotic gamma-aminobutyric acid in streptozotocin-induced diabetic mice

作     者：Amro Abdelazez Heba Abdelmotaal Smith Etareri Evivie Maha Bikheet Rokayya Sami Hassan Mohamed Xiangchen Meng Amro Abdelazez;Heba Abdelmotaal;Smith Etareri Evivie;Maha Bikheet;Rokayya Sami;Hassan Mohamed;Xiangchen Meng

作者机构：Key Laboratory of Dairy ScienceMinistry of EducationCollege of Food SciencesNortheast Agricultural UniversityHarbin 150030China College of Agriculture and ForestryLinyi UniversityLinyi 276005China Department of Dairy MicrobiologyAnimal Production Research InstituteAgriculture Research CentreDokkiGiza 12618Egypt Department ofMicrobiologySoilWaterEnvironmentand Microbiology Research InstituteAgriculture Research CentreGiza 12619Egypt Department of Food Science&Hunan NutritionFaculty of AgricultureUniversity of BeninBenin City 300001Nigeria Department of Dairy ScienceFaculty of AgricultureMinia UniversityMinia 61511Egypt Department of Food Science and NutritionCollege of SciencesTaif UniversityP.O.11099Taif21944Saudi Arabia Colin Ratledge Center for Microbial LipidsSchool of Agriculture Engineering and Food ScienceShandong University of TechnologyZibo 255049China Department of Botany and MicrobiologyFaculty of ScienceAl-Azhar UniversityAssiut 71524Egypt 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第1期

页      面：165-176页

核心收录：

学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：supported by grant from the National Key Research and Development Program of China(2018YFE0120500) 

主　　题：Diabetes Gamma-aminobutyric acid Postbiotics Lactobacillus brevis In vitro C57BL/6J mice 

摘      要：The therapeutic effect of gamma-aminobutyric acid(GABA)on diabetes was spread as one of the alarming epidemics *** study aims to investigate the function of Lactobacillus brevis KLDS_(1.0727) and KLDS_(1.0373) strains as glutamic acid decarboxylase 65(GAD65)carriers capable of generating GABA by comparing in vitro free and freeze-dried models and GABA intervention in *** amplification of gad and in vitro i.e.,(growth rate,viability at different pH,bile tolerance,and survivability in simulated gastric juice)were *** vivo experiments were conducted in 7 groups of C57BL/6J *** group was injected with streptozotocin(Cont_(STZ),INSSTZ,LAC1_(STZ),LAC_(1MFDSTZ),LAC_(2STZ),LAC_(2MFDSTZ))daily except for the control(Cont).One group was injected with insulin(INSSTZ).The body weight and hyperglycemia in the blood were assessed weekly,post-euthanasia blood plasma parameters,insulin,and histological examination were *** indicated *** strains demonstrated a great tolerance to bile and simulated gastric juice in vitro(P0.05).Cont_(STZ) had the highest average glucose level(6.84±6.46)mmol/L while INS_(STZ) expressed dramatically decreed in glucose level and displayed a significant decline in the average of weekly blood glucose(−5.74±3.08)mmol/*** lowest body weight(ContSTZ)was(19.30±0.25)*** on the blood plasma analysis,*** strains improved good cholesterol properties,liver and kidney functions,where most of these parameters fall within the average the reference range and prevent the development of symptoms of type 1 diabetes in *** recommended,*** should be commonly distributed as a postbiotic GABA in pharmaceutical and nutritional applications.