Enzymatic cascade systems for D-amino acid synthesis:progress and perspectives

作     者：Anwen Fan Jiarui Li Yangqing Yu Danping Zhang Yao Nie Yan Xu 

作者机构：School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of EducationJiangnan University1800 Lihu RoadWuxi 214122China State Key Laboratory of Food Science and TechnologyJiangnan University1800 Lihu RoadWuxi 214122China Suqian Industrial Technology Research Institute of Jiangnan UniversitySuqian 223814China 

出 版 物：《Systems Microbiology and Biomanufacturing》 (系统微生物学与生物制造（英文）)

年 卷 期：2021年第1卷第4期

页      面：397-410页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Financial supports from the National Natural Science Foundation of China(NSFC)(No.31872891) the 111 Project(No.111-2-06) the High-End Foreign Experts Recruitment Program(No.G20190010083) the National Program for Support of Top-Notch Young Professionals,the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,Top-Notch Academic Programs Project of Jiangsu Higher Education Institutions,the Jiangsu Province“Collaborative Innovation Center for Advanced Industrial Fermentation”Industry Development Program,the Program for the Key Laboratory of Enzymes of Suqian(No.M201803) the National First-Class Discipline Program of Light Industry Technology and Engineering(No.LITE2018-09)are greatly appreciated 

主　　题：D-amino acid Multi-enzyme system Cascade reaction Racemates resolution Asymmetric synthesis 

摘      要：D-amino acids,different from the ubiquitous L-amino acids,are recognized as the“unnaturalamino *** applications of D-amino acids have drawn increasing interest from researchers in recent years,and D-amino acids are widely used in various industries,including for food products,pharmaceuticals,and agricultural *** by the prevalent appli-cations,many synthetic methods for D-amino acids have been developed,which are mainly divided into chemical synthetic methods and biosynthetic *** synthesis of D-amino acids has a variety of disadvantages such as multiple reaction steps,low yields,low reaction rates,and difficulties in product ***,biosynthetic methods utilizing enzymes are attracting increasing attention because they are more energy-saving and environmentally friendly compared to traditional chemical *** all enzymatic methods,multi-enzymatic cascade catalytic methods have significant advantages,such as lower costs,no need for intermediate separation,and higher catalytic efficiency,which is ascribed to the spatial proximity of *** this review,advances in multi-enzyme cascade catalytic systems as well as chemo-enzymatic approaches to synthesize D-amino acids are discussed.