Identification of quantitative trait loci and candidate genes controlling seed pigments of rapeseed

作     者：ZHU Mei-chen HU Ran ZHAO Hui-yan TANG Yun-shan SHI Xiang-tian JIANG Hai-yan ZHANG Zhi-yuan FU Fu-you XU Xin-fu TANG Zhang-lin LIU Lie-zhao LU Kun LI Jia-na QU Cun-min ZHU Mei-chen;HU Ran;ZHAO Hui-yan;TANG Yun-shan;SHI Xiang-tian;JIANG Hai-yan;ZHANG Zhi-yuan;FU Fu-you;XU Xin-fu;TANG Zhang-lin;LIU Lie-zhao;LU Kun;LI Jia-na;QU Cun-min

作者机构：Chongqing Engineering Research Center for Rapeseed/College of Agronomy and BiotechnologySouthwest University/Academy of Agricultural SciencesSouthwest UniversityChongqing 400716P.R.China Agriculture and Agri-Food CanadaSaskatoon Research CentreSaskatoon S7N02XCanada 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第11期

页      面：2862-2879页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31830067) the Major S&T Projects on the Cultivation of New Varieties of Genetically Modified Organisms,China(2018ZX08020001) the Fundamental Research Funds for the Central Universities,China(XDJK2020B030) the China Agriculture Research System of MOF and MARA(CARS-12) the Natural Science Foundation of Chongqing,China(cstc2017jcyjAX0321) the Higher Education Discipline Innovation Project,China(B12006) 

主　　题：Brassica napus L. embryo seed coat pigments QTL 

摘      要：Rapeseed(Brassica napus L.)is an important source of edible vegetable oil and feed protein;however,seed pigments affect the quality of rapeseed oil and the feed value of the residue from oil ***,we used a population of rapeseed recombinant inbred lines(RILs)derived from the black-seeded male parent cultivar Zhongyou 821 and the yellow-seeded female parent line GH06 to map candidate genes controlling seed pigments in embryos and the seed *** detected 94 quantitative trait loci(QTLs)for seed pigments(44 for embryos and 50 for seed coat),distributed over 15 of the 19 rapeseed *** included 28 QTLs for anthocyanidin content,explaining 2.41–44.66% of phenotypic variation;24 QTLs for flavonoid content,explaining 2.41–20.26% of phenotypic variation;16 QTLs for total phenol content,accounting for 2.74–23.68% of phenotypic variation;and 26 QTLs for melanin content,accounting for 2.37–24.82% of phenotypic variation,indicating that these traits are under multigenic *** regions on chromosomes A06,A09 and C08 were associated with multiple seed pigment traits,including 15,19 and 10 QTLs,respectively,most of which were major QTLs explaining10% of the phenotypic *** on the annotation of the ***Darmor-bzhreference genome,67 candidate genes were predicted from these consensus QTLs regions,and 12 candidate genes were identified as potentially involved in pigment accumulation by RNA-seq and qRT-PCR *** preliminary results provide insight into the genetic architecture of pigment biosynthesis and lay a foundation for exploring the molecular mechanisms underlying seed coat color in ***.