Molecular phenotypes reveal heterogeneous engraftments of patient-derived hepatocellular carcinoma xenografts

作     者：Jianyong Zhuo Di Lu Jianguo Wang Zhengxing Lian Jiali Zhang Huihui Li Beini Cen Xuyong Wei Qiang Wei Haiyang Xie Xiao Xu Jianyong Zhuo;Di Lu;Jianguo Wang;Zhengxing Lian;Jiali Zhang;Huihui Li;Beini Cen;Xuyong Wei;Qiang Wei;Haiyang Xie;Xiao Xu

作者机构：Department of Hepatobiliary and Pancreatic SurgeryThe Center for Integrated Oncology and Precision MedicineAffiliated Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhou 310003China Department of Hepatobiliary and Pancreatic Surgerythe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China National Health Commission Key Laboratory of Combined Multi-organ TransplantationInstitute of Organ TransplantationZhejiang UniversityHangzhou 310003China 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2021年第33卷第4期

页      面：470-479页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from the National Science and Technology Major Project of China(No.2017ZX10203205) the National Natural Science Funds for Distinguished Young Scholar of China(No.81625003) Key Program National Natural Science Foundation of China(No.81930016) Key Research&Development Plan of Zhejiang Province(No.2019C03050) 

主　　题：Hepatocellular carcinoma patient-derived xenografts heterogeneous establishment molecular phenotype 

摘      要：Objective:Patient-derived xenograft(PDX)models provide a promising preclinical platform for hepatocellular carcinoma(HCC).However,the molecular features associated with successful engraftment of PDX models have not been ***:HCC tumor samples from 76 patients were implanted in immunodeficient *** molecular expression was evaluated by *** and tumor characteristics as well as tumor molecular expressions were compared for PDX engraftment using the Chi-square *** independent prediction parameters were identified by logistic regression ***:The engraftment rate for PDX models from patients with HCC was 39.47%(30/76).Tumors from younger patients and patients with elevated preoperative alpha-fetoprotein level had higher engraftment *** with poor differentiation and vascular invasion were related to engraftment *** positive expression of CK19,CD133,glypican-3(GPC3),and Ki67 in tumor samples was associated with engraftment *** regression analyses indicated that GPC3 and Ki67 were two of the strongest predictors of PDX *** with GPC3/Ki67 phenotypes showed heterogeneous engraftment rates,with 71.9%in GPC3^(+)/Ki67^(+)tumors,30.8%in GPC3^(-)/Ki67^(+)tumors,15.0%in GPC3^(+)/Ki67^(-)tumors,and 0 in GPC3^(-)/Ki67^(-)***:Successful engraftment of HCC PDXs was significantly related to molecular *** with the GPC3+/Ki67+phenotype were the most likely to successfully establish HCC PDXs.