Gene expression profile in immunologically injured liver cell of mice

作     者：HAN Fengmei CHENG Ming XIA Qisong CHEN Yong 

作者机构：Hubei Provincial Key Laboratory of Traditional Chinese Medicine & Biotechnology Faculty of Life Science Hubei University Wuhan 430062 China Hubei Provincial Key Laboratory of Traditional Chinese Medicine & Biotechnology Faculty of Life Science Hubei University Wuhan 430062 China Hubei Provincial Key Laboratory of Traditional Chinese Medicine & Biotechnology Faculty of Life Science Hubei University Wuhan 430062 China Hubei Provincial Key Laboratory of Traditional Chinese Medicine & Biotechnology Faculty of Life Science Hubei University Wuhan 430062 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2006年第49卷第5期

页      面：454-459页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：Technology Office of Hubei Province, (2003AA303B02) Major State Basic Research Development Program of China, (2002ccc00300) 

主　　题：mouse, immunological liver damage, cDNA microarray, gene expression profile. 

摘      要：To study the gene expression profiles between immunologically injured liver cell and normal liver cell of mice and to screen on a large scale the differentially expressed genes associated with the formation of liver injury,the experimental mice were randomly divided into the normal group for controlling and the immunologically liver-injured group induced by BCG and *** liver mRNA of the two groups were extracted respectively and reversely-transcribed to cDNA with the incorpora-tion of different fluorescence(Cy3,Cy5) labeled dUTP as the hybridization *** mixed probes were hybridized to the cDNA microarray *** fluorescent signal results were acquired by scanner ScanArray 4000 and analyzed with software GenePix Pro *** the 14112 target genes,293 genes were found to be significantly differentially expressed,in which 188 genes were up-regulated and 105 genes were *** on the analysis of biological functions of those differentially expressed genes,it was indicated that the occurrence and development of mouse liver damage induced by BCG and LPS were highly correlated with the processes of immune reac-tions,cell synthesis,metabolism,apoptosis and transportation in liver cell,which might be quite im-portant for elucidating the regulatory network of gene expression associated with the liver damage,also important for finally discovering the pathogenic mechanisms of immunological liver damage.