Optimization of Fermentation and Product Collection Process of the Functional Bacteria for Bioremediation of Shrimp Culture Environment

作     者：李秋芬 邹玉霞 辛福言 曲克明 陈民山 马绍赛 LI Qiufen;ZOU Yuxia;XIN Fuyan;QU Keming;CHEN Minshan;MA Shaosai

作者机构：中国水产科学研究院海洋食品安全与环境控制重点开放实验室黄海水产研究所中国水产科学研究院青岛266071 

出 版 物：《Marine Science Bulletin》 (海洋通报（英文版）)

年 卷 期：2006年第8卷第1期

页      面：53-60页

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：国家高技术研究发展计划(863计划)(819-2-07) NSFC(30200209) 

主　　题：Aquaculture environment Bioremediation Functional bacteria Fermentation 

摘      要：In order to implement industrial production of selected functional bacteria for bioremediation of shrimp culture environment, the fermentation conditions of the three functional bacteria, which have high ability in degrading organic pollutants at the bottom of shrimp ponds, were studied. The results showed that the favorable fermentation medium （per L） was 25 g of peptone, 5 g of yeast extract, and 0.2 g of ferric phosphate, and the initial pH value of the medium was 8.0. The optimum fermentation time was 20 h. The optimum stirring way was stirring one hour after one hour. Iso-electric point sedimentation collecting method was the most efficient and economic method to collect the bacteria cells after fermentation, with the optimum sediment pH of 3.67, 4.02, and 3.40 for the strains Lt7222, Gy7018 and Lt7511,respectively. It was also indicated that the survival and reproducing ability of the bacterial cells were not affected by the sedimentation process.