Comparison of LAMP,GeneXpert,Mycobacterial Culture,Smear Microscopy,***,TBAg/PHA Ratio for Diagnosis of Pulmonary Tuberculosis

作     者：Yan DENG Yi-fei DUAN Shu-pei GAO Jian-miao WANG Yan DENG;Yi-fei DUAN;Shu-pei GAO;Jian-miao WANG

作者机构：Department of Respiratory and Critical Care MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Infection ControlTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2021年第41卷第5期

页      面：1023-1028页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：pulmonary tuberculosis bronchoalveolar lavage fluid loop-mediated isothermal amplification GeneXpert TSPOT 

摘      要：Objective:To investigate the application value of loop-mediated isothermal amplification(LAMP),GeneXpert,mycobacterial culture,smear microscopy,***(TSPOT),ratio of TB-specific antigen to phytohemagglutinin(TBAg/PHA ratio)in the detection of mycobacterium tuberculosis in the bronchoalveolar lavage ***:A retrospective analysis was performed on the patients who underwent bronchoscopy from December 2018 to November 2019 in Tongji *** patients with positive tuberculosis culture or positive GeneXpert in bronchoalveolar lavage fluid were selected as the case group,and those without tuberculosis served as the control *** receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of LAMP,GeneXpert,culture,smear microscopy,TSPOT,and TBAg/PHA ***:For the patients with positive cultures as case,the sensitivity of LAMP,GeneXpert,smear microscopy,TSPOT and TBAg/PHA ratio was 73.49%,89.16%,25.30%,80.00%,33.85%,respectively,the specificity was 99.00%,100.00%,99.00%,86.00%,100.00%,respectively,the area under the ROC curve(AUC)was 0.849,0.938,0.633,0.830,0.669,*** the patients with positive GeneXpert as case,the sensitivity of LAMP,mycobacterial culture,smear microscopy,TSPOT and TBAg/PHA ratio was 73.20%,74.23%,22.68%,68.92%,29.73%,respectively,the specificity was 99.00%,100.00%,99.00%,86.00%,100.00%,respectively,the AUC was 0.853,0.878,0.623,0.775,0.649,***:The sensitivity of GeneXpert was *** sensitivity and diagnostic value of LAMP were slightly lower than those of GeneXpert,and were similar to tuberculosis *** sensitivity of smear microscopy was *** specificity of TSPOT was *** TBAg/PHA ratio0.2 was used as a diagnostic index,the specificity was improved,but the sensitivity was low.